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pLV-Puro_CI-hCOL4A5-Nluc_C29X

Plasmid

#198107

Purpose

lentiviral expression of hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C29X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_R1563X

Plasmid

#198109

Purpose

lentiviral expression of hCOL4A4_R1563Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1563X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_S36X

Plasmid

#198108

Purpose

lentiviral expression of hCOL4A4_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

S36X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc

Plasmid

#198106

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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L1-AA027

Plasmid

#185752

Purpose

Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

HygR2

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMCh-1478

Plasmid

#82424

Purpose

Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Tags

GST

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1547

Plasmid

#82434

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO1

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CROP-seq-opti

Plasmid

#106280

Purpose

A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

Plasmid

#180587

Purpose

FdCd

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Cre

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Caspase-Reporter

Plasmid

#243673

Purpose

Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

Depositor

Matthias Wirth

Article

Selcen et al Cell Death Discov, 11, 368, 06 August 20

Insert

Split-GFP with DEVD sequence and T2A mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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SpRY Cas9

Plasmid

#188491

Purpose

Expresses FLAG tagged SpRY Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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