We narrowed to 5,835 results for: pcas

1. pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA

   Plasmid

   #108723

   Purpose

   Expresses N-terminus to aa229 of Cre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa229
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA

   Plasmid

   #108724

   Purpose

   Expresses aa230 to C-terminus of Cre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa230 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

   Plasmid

   #108729

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS puro human-GFP-RLIM-IRES-mCherry

   Plasmid

   #232243

   Purpose

   Mammalian expression of RLIM-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   RLIM-GFP-IRES-mCherry (RLIM Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

   Plasmid

   #232240

   Purpose

   Mammalian expression of REX1[degron]-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   REX1 (aa 70-85)

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107320

   Purpose

   Expresses SpCas9 fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

   Plasmid

   #108730

   Purpose

   Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa271 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

   Plasmid

   #200277

   Purpose

   Characterisation of the sialic acid-responsive circuit with fluorescent reporter

   Depositor

   Matthew Chang

   Article

   Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

   Inserts

   CadC
   NanR
   

   Expression

   Bacterial

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

   Plasmid

   #108748

   Purpose

   Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa28 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65455

    Purpose

    Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid

    #223115

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

    Plasmid

    #179005

    Purpose

    Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

    Plasmid

    #179004

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36880

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    insulator
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid

    #140196

    Purpose

    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta) , NLS

    Promoter

    PgpdA and PtrpC

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid

    #140199

    Purpose

    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Promoter

    PgpdA

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

    Plasmid

    #168216

    Purpose

    Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    SV40 NLS SpCas9
    

    Use

    Lentiviral

    Tags

    HA, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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