We narrowed to 6,334 results for: nls
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Plasmid#82470PurposeExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cellsDepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-myc-mCE
Plasmid#82459PurposeExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cellsDepositorAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
SepOTSι
Plasmid#68290PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EFSep21, 2xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSη
Plasmid#68288PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 2xtRNA-Sep
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSζ
Plasmid#68287PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 2xtRNA-Sep
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSδ
Plasmid#68285PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 1xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pMXs-I2
Plasmid#48880PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterLTRAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Sgf73(1-106)/PET32a
Plasmid#26962DepositorInsertSgf73(1-106) (SGF73 Budding Yeast)
TagsHis6 and TrxExpressionBacterialMutationwith TEV site to cleave the N-terminal his+Trx tagAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWChe(#488)
Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)MutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-BAT-RED
Plasmid#20674DepositorInsertWnt reporter dsRED
TagsNLSExpressionBacterialAvailable SinceMay 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB2
Plasmid#228868PurposePE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS-2A-MLH1-SB
UseCRISPRAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-EGFP
Plasmid#89875PurposeEGFP reporter vector of iTango system for AAV productionDepositorInserttetO-EGFP
UseAAVExpressionMammalianPromotertetOAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only