We narrowed to 3,387 results for: psin

1. HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

   Plasmid

   #178825

   Purpose

   Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   QuasAr6b_citrine
   

   Use

   Cre/Lox and Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQCXIP-SHMY-A1Pi

   Plasmid

   #182648

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   A1Pi (SERPINA1 Human)
   

   Use

   Retroviral

   Tags

   His6-tag, N-terminal ER targeting sequence of hem…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174646

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   CMV (with TetOn)

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

   Plasmid

   #174635

   Purpose

   Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pB80-KIF1A(1-383)-GFP-SSPB(micro)

   Plasmid

   #174627

   Purpose

   Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

   Plasmid

   #160069

   Purpose

   Conditionally overexpress collybistin (CB2SH3-) and mCitrine

   Depositor

   Angel de Blas

   Article

   George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   AAV

   Tags

   HA- in CB2SH3

   Expression

   Mutation

   Promoter

   human Synapsin promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLuc-OptoJNKi

   Plasmid

   #89744

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cry2 mCherry GRK2 ct in pcDNA3.1

   Plasmid

   #64212

   Purpose

   mCherry fused between Cry2 and GRK2ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK2 ct (GRK2 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and cmv

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGP-AAV-syn-jGCaMP8m-WPRE

   Plasmid

   #162375

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-iFlpV

    Plasmid

    #140137

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iFlpV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-ChrimsonR-tdT

    Plasmid

    #59171

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV hSYN DIO mGluR1-myc-FLAG

    Plasmid

    #166227

    Purpose

    AAV expressing cre-dependent mGluR1-myc-FLAG

    Depositor

    Jason Aoto

    Article

    Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

    Insert

    metabotropic glutamate receptor 1 (Grm1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DiLiCre 2.0

    Plasmid

    #198752

    Purpose

    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

    Depositor

    Jacco van Rheenen

    Article

    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

    Insert

    Cre-PhoCI-ERT2-ERT2
    

    Use

    Lentiviral

    Tags

    Cre, kept in a photocleavable site, followed by 2…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge