270,948 results

1. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-mGold2s-Calnexin-N-14

   Plasmid

   #231768

   Purpose

   Visualization of ER in mammalian cells using mGold2s

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2s-Calnexin-N-17 (CANX Human)
   

   Tags

   mGold2s

   Expression

   Mammalian

   Mutation

   I474N

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SypHer2-DAAO-NES

   Plasmid

   #119184

   Purpose

   Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   SypHer2-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SypHer2-DAAO-NES

   Plasmid

   #119184

   Purpose

   Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   SypHer2-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAK184

   Plasmid

   #167281

   Purpose

   MazF-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Promoter

   Pxyl

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

   Plasmid

   #108912

   Purpose

   Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Has Service

   AAV9

   Insert

   ChroME-ST
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

   Plasmid

   #108912

   Purpose

   Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Has Service

   AAV9

   Insert

   ChroME-ST
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_C_EPHB2

    Plasmid

    #187797

    Purpose

    MAC-tagged gene expression of human EPHB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB2 (EPHB2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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