We narrowed to 6,157 results for: RIK

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Lenti-sgSis#2/Cre

Plasmid

#193238

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#2 (Sis Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSis#1/Cre

Plasmid

#193237

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#1 (Sis Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZfp536#2/Cre

Plasmid

#193249

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgZfp536#2 (Zfp536 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#2/Cre

Plasmid

#193224

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#2 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst1-3xHA

Plasmid

#183540

Purpose

Express Dcst1-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRbm10#2/Cre

Plasmid

#173651

Purpose

Expresses a Rbm10-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rbm10 (Rbm10 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRnf43#2/Cre

Plasmid

#173614

Purpose

Expresses a Rnf43-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rnf43 (Rnf43 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSetd2#2/Cre

Plasmid

#173615

Purpose

Expresses a Setd2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Setd2 (Setd2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRnf43#1/Cre

Plasmid

#173613

Purpose

Expresses a Rnf43-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rnf43 (Rnf43 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf1#2/Cre

Plasmid

#173608

Purpose

Expresses a Nf1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf1 (Nf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.DMAP1

Plasmid

#184651

Purpose

Retroviral expression of mouse Dmap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 Del EC2

Plasmid

#184025

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf1#1/Cre

Plasmid

#173607

Purpose

Expresses a Nf1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf1 (Nf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Slc1a3-CreERT2 Targeting Vector

Plasmid

#129409

Purpose

Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Slc1a3 nickase 2

Plasmid

#129411

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA targeting Slc1a3 (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Armc12/FLAG

Plasmid

#180494

Purpose

Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-FLAG

Plasmid

#179921

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-1D4

Plasmid

#179922

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Tags

1D4

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Cdipt-V5

Plasmid

#175144

Purpose

Lentiviral expression of mouse Cdipt-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Cdipt (Cdipt Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-mVenus-SSPB(micro)

Plasmid

#174635

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(micro)

Plasmid

#174627

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

Plasmid

#174712

Purpose

Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Mutation

mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434)

Plasmid

#174713

Purpose

Mammalian expression of mouse Meikin with inserted C-terminal Separase cleavage site inactivated

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Mutation

mMeikin: 1-391; hRad21: 142-275, E169R, R172E; mM…

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔEC gRNA resistant

Plasmid

#163413

Purpose

Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The extracellular domain (27-150 aa) is deleted. …

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔTail gRNA resistant

Plasmid

#163412

Purpose

Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The C-terminal tail domain (530-1038 aa) is delet…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-f1(+)-TOMM20-dTomato

Plasmid

#115914

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato (TOMM20 Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EN1680_Sororin-Cterm

Plasmid

#156451

Purpose

For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

Cas9, SORORIN (Cdca5 Mouse)

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shCenpf

Plasmid

#160956

Purpose

Cenpf shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cenpf shRNA (Cenpf Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_Plk5(300-595) PBD domain

Plasmid

#136316

Purpose

Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Expression

Mammalian

Mutation

fragment 300-595

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (G112P)

Plasmid

#135496

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

G112P

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-A2-VN (L156P)

Plasmid

#135497

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

L156P

Promoter

CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE:mTREK-1_cryst

Plasmid

#133271

Purpose

X. laevis expression vector. It will generate the TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetBl-CLIP-Cep97

Plasmid

#136849

Purpose

Mammalian expression of the centrosomal protein Cep97 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep97 (CEP97 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-N-Flag-Snrnp40-skywarp

Plasmid

#134250

Purpose

Lentivector encoding Flag-tagged skywarp form of Snrnp40 (missing exon 5)

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 179-219

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)

Plasmid

#135501

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-A2-VN (I52P)

Plasmid

#135486

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

I52P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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