We narrowed to 13,220 results for: CRISPR-Cas9

1. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

   Plasmid

   #193048

   Purpose

   Heatshock expressed Cas9

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp16.41p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL-gRNA(anti-cGAS)-Cas9-Puro

   Plasmid

   #130908

   Purpose

   gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

   Plasmid

   #192131

   Purpose

   Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

   Plasmid

   #169596

   Purpose

   Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

   Plasmid

   #101104

   Purpose

   Encodes dCas9(N) fused to VEGFR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101114

   Purpose

   Encodes dCas9(N) fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101106

    Purpose

    Encodes FKBP-dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-FKBP-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and tp1

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

    Plasmid

    #227774

    Purpose

    Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    loxP-dsR-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-MMLVgag-3xNES-Cas9

    Plasmid

    #181752

    Purpose

    MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLV-Gag-3xNES-Cas9 (Spy)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BICstim-Gag-dCAS9-VPR

    Plasmid

    #120922

    Purpose

    encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

    Depositor

    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG (FMLV)-dCAS9-VPR
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-GfABC1D-SaCas9-WPRE3-pA

    Plasmid

    #203540

    Purpose

    SaCas9 expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pED9x (dCas9-KRAB-mCherry)

    Plasmid

    #163956

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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