We narrowed to 49,358 results for: Nes;

1. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA GAL4 CREB 1-341 M1

   Plasmid

   #22973

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

   Insert

   CREB (Creb1 GAL4-fusion, Rat)
   

   Use

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   Changed Serine 133 to Alanine of CREB

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGG-PIP56

   Plasmid

   #196794

   Purpose

   Contains PR1 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   PATHOGENESIS-RELATED GENE 1 (PR1 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-Pav-GFP

   Plasmid

   #24286

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Pavarotti (pav Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-Pav-GFP

   Plasmid

   #24286

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Pavarotti (pav Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

8. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

9. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307 GFP-Kif26b-C

    Plasmid

    #118483

    Purpose

    lentiviral expression of GFP-Kif26b-C in mammalian cells

    Depositor

    Henry Ho

    Article

    Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

    Insert

    mouse Kif26b-C (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Encodes GFP fused to amino acid 1735 to 2112 of m…

    Promoter

    EF1A

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FXYD2-luc

    Plasmid

    #31056

    Depositor

    Gerhart Ryffel

    Article

    Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

    Insert

    FXYD2 (FXYD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    FXYD2 promoter from -2382 to -1

    Promoter

    Available since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-DroshaS300/302A

    Plasmid

    #62530

    Purpose

    Serines 300 and 302 of Drosha are mutated to alanines

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serines 300 and 302 to alanines

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307 GFP-Kif26b-C

    Plasmid

    #118483

    Purpose

    lentiviral expression of GFP-Kif26b-C in mammalian cells

    Depositor

    Henry Ho

    Article

    Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

    Insert

    mouse Kif26b-C (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Encodes GFP fused to amino acid 1735 to 2112 of m…

    Promoter

    EF1A

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FXYD2-luc

    Plasmid

    #31056

    Depositor

    Gerhart Ryffel

    Article

    Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

    Insert

    FXYD2 (FXYD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    FXYD2 promoter from -2382 to -1

    Promoter

    Available since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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