Skip to main content
Addgene

We narrowed to 2,788 results for: aen

Showing: 2701 - 2720 of 2788 results
  1. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

    Plasmid
    #197599
    Purpose
    Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive
    Insert
    Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    Use
    Tags
    Expression
    Worm
    Mutation
    D387A, E490G
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid
    #209846
    Purpose
    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
    Insert
    STX5 (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. mGFP-hNET-A457P

    Plasmid
    #193365
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Insert
    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    Use
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    A457P (GCC to CCC)
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. mGFP-hNET-R121A/K334A/R440A

    Plasmid
    #193366
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Insert
    human Norepinephrine Transporter (SLC6A2 Human)
    Use
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid
    #154845
    Purpose
    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
    Insert
    Stx5 (Short isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Lacks amino acids 1-54, enocdes short isoform of …
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid
    #203797
    Purpose
    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid
    #203798
    Purpose
    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    Available since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid
    #187936
    Purpose
    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327
    Depositor
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Use
    Tags
    GST-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 2701 - 2720 of 2788 results