We narrowed to 6,890 results for: nls

1. pCMV-BE1

   Plasmid

   #73019

   Purpose

   Expresses BE1 with C-terminal NLS in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. enOgeuIscB with ωRNA-v2

   Plasmid

   #220939

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SupD v2

   Plasmid

   #198598

   Purpose

   Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SupD
   

   Expression

   Bacterial

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT15489_dABE8e

   Plasmid

   #174127

   Purpose

   plasmid expressing ABE8e with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ149-pcDNA3-BhCas12b_v4

   Plasmid

   #122446

   Purpose

   Expresses BhCas12b_v4 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   CMV-NLS-BhCas12b_v4-NLS-3xHA
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT15482_ABE8e

   Plasmid

   #174120

   Purpose

   Expresses ABE8e with an EF1a promoter

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   eABE
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1325

    Plasmid

    #111132

    Purpose

    Expresses USP1.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    USP1 (USP1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Tags

    3XHA

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. JAB2296

    Plasmid

    #190365

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt
    

    Expression

    Plant

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. XW52

    Plasmid

    #65838

    Purpose

    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Czipper-QF-AD-NLS
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Pmig-13

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. JAB3312

    Plasmid

    #190406

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
    

    Expression

    Plant

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP E17TAG/Q157TAG

    Plasmid

    #68299

    Purpose

    E17/Q157 TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    GFP E17TAG/Q157TAG
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge