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We narrowed to 6,336 results for: puc

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Showing: 2701 - 2720 of 6336 results

  4. HR-TERT-SV40-GFP

    Plasmid
    #71397
    Purpose
    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Tags
    SV-40 GFP insertion in TERT promotor
    Promoter
    Endogenous TERT promotor
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71397-plasmid-map-sequence-id-128453

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  10. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  11. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124810-plasmid-map-sequence-id-243698

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  12. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available Since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

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  13. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available Since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61576-plasmid-map-sequence-id-209910

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  14. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available Since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

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Showing: 2701 - 2720 of 6336 results