We narrowed to 32,166 results for: uros

1. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

   Plasmid

   #163177

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN-puro-P2A-FRB-Fis1

   Plasmid

   #135297

   Purpose

   stably express FBR-Fis1 in mammalian cells with puromycin selection

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   FRB-Fis1 (FIS1 Human)
   

   Use

   Retroviral

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-puro-CLIP-FLAG-CD59

   Plasmid

   #50375

   Purpose

   Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   CLIP
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. integrin alpha9 EGFP pBabe puro

   Plasmid

   #13605

   Depositor

   Dean Sheppard

   Insert

   integrin alpha 9 (ITGA9 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro-HA-PIK3CA(H1047R;G320E)

   Plasmid

   #33254

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and glycine 32…

   Promoter

   SV40

   Available Since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGL3-sgACSL3.C-Cas9-P2A-Puro

   Plasmid

   #129412

   Purpose

   Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgACSL3.C (ACSL3 Human)
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPER retro puro macroH2A2 shRNA

   Plasmid

   #30518

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   mH2A2 shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-U6-sgRNA-PGK-puromycin-AflII

   Plasmid

   #106404

   Purpose

   Empty guide RNA cloning vector with an AflII site added from Addgene 41824

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid

    #171801

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. H446 EF1a RA-ABE6.3 p2A Puro

    Plasmid

    #139104

    Purpose

    Lentiviral expression of ABE6.3.

    Depositor

    Wen Xue

    Article

    Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

    Insert

    ABE RA6.3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. integrin beta6 Y774F pBabe puro

    Plasmid

    #13599

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 Y774F (ITGB6 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Y774F

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-2xFLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172622

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid

    #192585

    Purpose

    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    Lentiviral

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYJ21-lenti-EF1a-ASAP2f-puro

    Plasmid

    #131065

    Purpose

    Forced expression of ASAP2f

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

    Insert

    ASAP2f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid

    #185370

    Purpose

    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF

    Depositor

    Ayaz Najafov

    Insert

    ARAF (ARAF Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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