We narrowed to 144,006 results for: lias

1. pcDNA5-FRT-TO-EGFP-RIP2_S176E

   Plasmid

   #131203

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

   Insert

   RIPK2 S176E (RIPK2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S176E

   Promoter

   pCMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT-TO-EGFP-RIP2_Y474F

   Plasmid

   #131204

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

   Insert

   RIPK2 Y474F (RIPK2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y474F

   Promoter

   pCMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hC73S-HA

   Plasmid

   #111529

   Purpose

   Expresses human KCNB1 C73S in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

   Insert

   human C73S-Kv2.1 + HA (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C73S

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hC73A-HA

   Plasmid

   #111526

   Purpose

   Expresses human KCNB1 C73A in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

   Insert

   human C73A-Kv2.1 + HA (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C73A

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mSTIM1_1-250-CFP

   Plasmid

   #69552

   Purpose

   Expresses a STIM1_1-250 fragment mammalian cells

   Depositor

   Yubin Zhou

   Article

   Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

   Insert

   mSTIM1_1-250
   

   Use

   Tags

   Cyan Fluorescent Protein (CFP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET-Ce25

   Plasmid

   #51296

   Purpose

   Expresses a plant arginyl-tRNA synthetase in E.coli

   Depositor

   Gabor Igloi

   Article

   Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7

   Insert

   arginyl-tRNA synthetase
   

   Use

   Tags

   His Tag and Thioredoxin

   Expression

   Bacterial

   Mutation

   GTT (Val) insertion after initiation codon to per…

   Promoter

   T7

   Available since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcdna3 EE Jip1b

   Plasmid

   #52127

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
   

   Use

   Tags

   EE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEBB-3XMyc-TRAF2 S327A

   Plasmid

   #44108

   Depositor

   William Hahn

   Article

   Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

   Insert

   TRAF2 S327A (TRAF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Serine 327 to Alanine

   Promoter

   EF1α

   Available since

   May 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mouse E-cadherin GFP (423)

    Plasmid

    #67937

    Purpose

    Mammalian expression of E-cadherin-GFP

    Depositor

    Alpha Yap

    Article

    Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-His:CRY2:nls:VPR

    Plasmid

    #135989

    Purpose

    Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Inserts

    CRY2 domain fused to the VPR activation domain
    CRY2 domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFP-Rab8a-QL

    Plasmid

    #86076

    Purpose

    A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-PIF3-mEGFP

    Plasmid

    #100283

    Purpose

    Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N-terminus (1-100 aa) of PIF3 is used

    Promoter

    CAG promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-Rab8a-TN

    Plasmid

    #86077

    Purpose

    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-Ago2

    Plasmid

    #21538

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    The first 7aa of this Ago2 protein are different …

    Promoter

    Available since

    Sept. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BSD-CryC

    Plasmid

    #209672

    Purpose

    Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    Split-TurboID-C and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-WHaloCaMP1a-EGFP

    Plasmid

    #205313

    Purpose

    Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UTS2R-DuET

    Plasmid

    #213386

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    UTS2R (UTS2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNEU-hMbPylRS-4xU6M15

    Plasmid

    #105830

    Purpose

    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

    Depositor

    Irene Coin

    Article

    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

    Inserts

    Humanized MbPylRS
    tRNAM15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (Y271A, Y349F)MbPylRS

    Promoter

    CMV and U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HDAC6 Flag

    Plasmid

    #13823

    Purpose

    Mammalian expression of human histone deacetylase 6 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-HsPeg10rc3

    Plasmid

    #174859

    Purpose

    Expresses recoded human Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Homo sapiens PEG10 (PEG10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-Hs.cargo(Cas9)

    Plasmid

    #174864

    Purpose

    Expresses human Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by HsPeg10 UTRs
    

    Use

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV- δLight

    Plasmid

    #201224

    Purpose

    Express deltaLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    δLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge