We narrowed to 5,339 results for: 290

1. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104095

   Purpose

   Expresses TREAT-siRNA reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV Cbx5-V5

   Plasmid

   #175105

   Purpose

   Lentiviral expression of mouse Cbx5-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Cbx5 (Cbx5 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1_MeCP2(R306C)

   Plasmid

   #110188

   Purpose

   Expression of mouse MeCP2 (R306C) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arg 306 Cys

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104094

   Purpose

   Expresses TREAT reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

   Expression

   Mammalian

   Mutation

   Promoter

   TetCMV

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-NSD3-short

   Plasmid

   #72552

   Purpose

   expresses 3*FLAG tagged human NSD3-short

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   NSD3-short (NSD3 Human)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   LTR

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. bio-CD4d3+4-Kell blood group

   Plasmid

   #73089

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Kell blood group (KEL Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104096

   Purpose

   Expresses TREAT-ARE reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1_MeCP2(deltaNC)

   Plasmid

   #110190

   Purpose

   Expression of mouse MeCP2 (deltaNC) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta N and C termini

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Agarabi CHO Heavy Chain in pJ201

    Plasmid

    #111567

    Purpose

    Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Human heavy chain
    

    Use

    Synthetic Biology; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MED4

    Plasmid

    #15425

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED4 (MED4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pLV Gpat4-V5

    Plasmid

    #175145

    Purpose

    Lentiviral expression of mouse Gpat4-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Gpat4 (Gpat4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB112

    Plasmid

    #180173

    Purpose

    pAAV construct GluN1WT (shRNA resistant)

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN1 (Grin1 Rat)
    

    Use

    AAV

    Tags

    Phluorin

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTet-O-KLF7-T2A-PuroR

    Plasmid

    #162346

    Purpose

    Lentiviral expression of KLF7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MED10

    Plasmid

    #15417

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED10 (Med10 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. p-mCherry-N1-iChloC_CA

    Plasmid

    #98171

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChloC_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, C128A, T159C, D156N

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPR55-DuET

    Plasmid

    #213285

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR55 (GPR55 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

    Plasmid

    #124865

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid

    #178224

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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