We narrowed to 31,305 results for: son
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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro
Plasmid#168807PurposePiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expressionDepositorInsertMYOD1 (MYOD1 Synthetic, Human)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 BLAST
Plasmid#164228Purposelentiviral plasmid for GFP-hTREX1DepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gsi (Ser)
Plasmid#109352PurposeGsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGsi chimera
TagsNoneExpressionMammalianMutationChanged Cys at position 391 to Serine (Pertussis …PromoterCAGAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST
Plasmid#208046PurposeEnables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidinDepositorAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike-d18
Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
ExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2
Plasmid#110055PurposeCry1 transcription fluorescence reporterDepositorInsertCry1 promoter and Venus (Cry1 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterCry1Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A(H188A)-polyA
Plasmid#101052Purposein vitro transcription (T7 promoter)DepositorAvailable SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-AT1R-APEX
Plasmid#96847PurposeFor expression of AT1R-APEX in mammalian cellsDepositorInsertAT1R-APEX (AGTR1 Human)
ExpressionMammalianAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2
Plasmid#110058PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and mCherry and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterPer2Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEM040 [Multiome Perturb-seq sgRNA expression vector]
Plasmid#224898PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experimentsDepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
ExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA
Plasmid#156447PurposeFor transient expression of mouse SMC3 tagged with mKate2DepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL7 lenti-dcas9-3xflag-blast
Plasmid#112133Purposelenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)DepositorInsertdCas9(D10A, N863A)-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A mutants in Cas9PromoterEF1aAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only
