We narrowed to 11,219 results for: NSI

1. pPAct2noUTR (GB0192)

   Plasmid

   #68177

   Purpose

   Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 promoter (no UTR)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGAL4-DBD-RUNX2wt-IDR

   Plasmid

   #145252

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2Awt-IDR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWP1_mFTCD

   Plasmid

   #102317

   Purpose

   expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Ftcd (Ftcd Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BAS289 C terminal E2C G418R

   Plasmid

   #74084

   Purpose

   S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   E2Crimson
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHH0103_NCK2-3/3

   Plasmid

   #91256

   Purpose

   Protein expression and purification of human SH3 domain construct NCK2-3/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCK2-3/3 (NCK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_SHANK1-1/1

   Plasmid

   #91517

   Purpose

   Protein expression and purification of human SH3 domain construct SHANK1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SHANK1-1/1 (SHANK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-RlACR_477-EYFP

   Plasmid

   #103771

   Purpose

   Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R1ACR_477
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TU#1806_CRISPR_unc-73_exon21

   Plasmid

   #82360

   Purpose

   to create unc-73E null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJOE6089.4

   Plasmid

   #135081

   Purpose

   pBR322-based L-rhamnose-inducible vector.

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

   Insert

   P-rhaP-BAD-eGFP-Strep-tag II
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDsgRNA-malG

    Plasmid

    #89951

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    malG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. As1

    Plasmid

    #123159

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J117, ParsR, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUWR-Cad-mTFP

    Plasmid

    #58327

    Purpose

    Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-mTFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pHH0103_PTK6-1/1

    Plasmid

    #91441

    Purpose

    Protein expression and purification of human SH3 domain construct PTK6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PTK6-1/1 (PTK6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CaM-RhoA(DP)-Venus

    Plasmid

    #31994

    Depositor

    Kevin Truong

    Article

    Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

    Insert

    CaM-RhoA(DP) (RHOA Human)
    

    Use

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Q63L mutation in RhoA, E7S and D65Y in CaM

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pKDsgRNA-hisG

    Plasmid

    #89954

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    hisG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHcPro (GB0067)

    Plasmid

    #68196

    Purpose

    Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    TEV HcPro coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_SH3RF1-1/4

    Plasmid

    #91544

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF1-1/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF1-1/4 (SH3RF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_SH3RF1-2/4

    Plasmid

    #91457

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF1-2/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF1-2/4 (SH3RF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_SH3RF1-4/4

    Plasmid

    #91374

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF1-4/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF1-4/4 (SH3RF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_SH3RF3-2/2

    Plasmid

    #91356

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF3-2/2 (SH3RF3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_SH3RF1-3/4

    Plasmid

    #91366

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF1-3/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF1-3/4 (SH3RF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_SH3RF3-1/2

    Plasmid

    #91280

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF3-1/2 (SH3RF3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pXPR_BRD003 sgLacZ-1

    Plasmid

    #74968

    Purpose

    sgLacZ-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXPR_BRD003 sgLacZ-2

    Plasmid

    #74969

    Purpose

    sgLacZ-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BAS291 C terminal mKO2 kanR

    Plasmid

    #74086

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

26. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_SH3BP4-1/1

    Plasmid

    #91532

    Purpose

    Protein expression and purification of human SH3 domain construct SH3BP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3BP4-1/1 (SH3BP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_SORBS2-1/3

    Plasmid

    #91387

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS2-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS2-1/3 (SORBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_VAV1-1/2

    Plasmid

    #91355

    Purpose

    Protein expression and purification of human SH3 domain construct VAV1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV1-1/2 (VAV1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMM549

    Plasmid

    #112531

    Purpose

    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BAS290 C terminal E2C natR

    Plasmid

    #74085

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pHH0103_STAM-1/1

    Plasmid

    #91428

    Purpose

    Protein expression and purification of human SH3 domain construct STAM-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAM-1/1 (STAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBad-CA TAG233

    Plasmid

    #105838

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 233

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sh-Myocardin

    Plasmid

    #100768

    Purpose

    expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    sh-Myocardin
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_PACSIN3-1/1

    Plasmid

    #91335

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN3-1/1 (PACSIN3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-RsACR_995-EYFP

    Plasmid

    #103772

    Purpose

    Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin RsACR_995
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-GcACR_457-EYFP

    Plasmid

    #103137

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_457
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. M26V DJ-1 pET15b

    Plasmid

    #60688

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26V DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26V

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only