We narrowed to 5,339 results for: 290

1. pCALNL-mutKir2.1

   Plasmid

   #125579

   Purpose

   Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A non-conducting mutant (GYG -> AAA).

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF712-pET28a-Hs-delta44-GCDH-NHis wt

   Plasmid

   #85113

   Purpose

   expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta44) GCDH (GCDH Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   silent SNP: G1041T

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

   Plasmid

   #163719

   Purpose

   Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP-Hyg-CTCF-Nterm-N1

   Plasmid

   #131800

   Purpose

   Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 796-2181 (the N-terminus is l…

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_HA.NWS.Ollas_mCherry-NLS

   Plasmid

   #178263

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.NWS.Ollas and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-mCherry-C1-Phobos

   Plasmid

   #98166

   Purpose

   blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-mCherry-C1-Aurora

   Plasmid

   #98167

   Purpose

   red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_NWS.HA.V5_mCherry-NLS

   Plasmid

   #178283

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.HA.V5 and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-eDHFR-Cdc42Q61L

   Plasmid

   #107267

   Purpose

   mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ET8-GPIba-6His

    Plasmid

    #102878

    Purpose

    Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus

    Depositor

    Timothy Springer

    Article

    Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1

    Insert

    Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid

    #178234

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.S.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_C.HA.V5_mCherry-NLS

    Plasmid

    #178242

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-TetON-mEGFP-NGN2_WT

    Plasmid

    #215610

    Purpose

    For integration of NGN2 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_ASCL3_P2A_Hygro_Barcode

    Plasmid

    #120428

    Purpose

    Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL3 (ASCL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSicoR-sh4EBP2

    Plasmid

    #81121

    Purpose

    expresses an shRNA targeting murine 4E-BP2

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4E-BP2 shRNA (Eif4ebp2 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF713-pEGFP-N1-Hs-FL-METTL20 wt

    Plasmid

    #85114

    Purpose

    transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-tetpA-hNEUROG2-iRPT

    Plasmid

    #140764

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    NEUROG2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

    Plasmid

    #104566

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-p65
    

    Use

    Tags

    3x HA and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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