We narrowed to 8,696 results for: Ski

1. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

   Plasmid

   #120565

   Purpose

   Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, T35A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

   Plasmid

   #120567

   Purpose

   Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

   Plasmid

   #120566

   Purpose

   Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHR-GFP-FUSN-VP16

   Plasmid

   #183933

   Purpose

   Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-hACE2-hygro

   Plasmid

   #161758

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJEP313-pAAV-CMV-SaCas9-DIO-pA

   Plasmid

   #113690

   Purpose

   A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET iGluSnFR3 v82

    Plasmid

    #175187

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v82
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82706

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast POLE1

    Plasmid

    #160802

    Purpose

    Express POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Psicheck Cdk2ap1Exon1

    Plasmid

    #178028

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

    Plasmid

    #120561

    Purpose

    Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 SARS-CoV-2 S D614G

    Plasmid

    #158075

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

    Plasmid

    #120559

    Purpose

    Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-EGFP MASTL WT (siRNA resistant)

    Plasmid

    #191011

    Purpose

    Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE-TAZ-CAMTA1

    Plasmid

    #235680

    Purpose

    Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

    Depositor

    Duojia Pan

    Article

    Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

    Insert

    TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-ALDOA_sgRNA1

    Plasmid

    #201590

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82704

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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