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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FRT-EF1a-TVA-2A-N2cG

Plasmid

#172361

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1

Plasmid

#124651

Purpose

Soma-localized ChrimsonR for dense expression in excitatory neurons

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV1 and AAV9

Insert

ChrimsonR

Use

AAV

Tags

mScarlet

Expression

Mammalian

Promoter

CamKIIa

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-BEC enhancer-minBG-SYFP2

Plasmid

#236722

Purpose

SYFP2-tagged Brain Endothelial Cell Enhancer mini Beta Goblin promoter

Depositor

Xiangmin Xu

Article

Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

Type

Empty backbone

Use

AAV

Tags

SYFP2

Promoter

minimal beta globulin

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-EYFP-WPRE

Plasmid

#154870

Purpose

Human synapsin-1 promoter; Flp-dependent EYFP

Depositor

Ulrik Gether

Insert

EYFP

Use

AAV and Mouse Targeting; Flp-frt

Expression

Mammalian

Promoter

Human synapsin promoter

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jRex-GECO

Plasmid

#172714

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jRex-GECO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mGFP-APEX2

Plasmid

#171936

Purpose

Reporter for correlated light and electron microscopy.

Depositor

David Berson

Article

Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

Insert

mGFP-APEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-WPRE

Plasmid

#51505

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

Plasmid

#210391

Purpose

AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

Depositor

Benjamin Kleinstiver

Article

Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

Insert

AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CamKII promoter for Cre, human U6 promoter for Sp…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry-IRES-Cre

Plasmid

#55632

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Inserts

mCherry
Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2-WPRE

Plasmid

#218874

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro-mScarlet

Plasmid

#227274

Purpose

AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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