We narrowed to 3,387 results for: psin

1. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

   Plasmid

   #174633

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
   

   Use

   Tags

   mCherry-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROF346

   Plasmid

   #155331

   Purpose

   Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BM00369

   Plasmid

   #155338

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

   Plasmid

   #209851

   Purpose

   Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

   Depositor

   Volker Busskamp

   Article

   Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

   Insert

   light-driven sodium pump ErNaR
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LIC1B_Caspase-LOVN7C-7 C450A

   Plasmid

   #104628

   Purpose

   bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

   Depositor

   Jim Wells

   Article

   Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Caspase-3 (CASP3 Human)
   

   Use

   Tags

   His and LOV2

   Expression

   Bacterial

   Mutation

   C450A (dark-locked and light-insensitive mutation)

   Promoter

   T7

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-hTRF2-tagRFP-T

   Plasmid

   #103812

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hTRF2: deletion of amino acids 1-42 and 476 compa…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2clust-mCherry-RANK-P2A-CIBNcaax

   Plasmid

   #208613

   Purpose

   Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

   Depositor

   Tomohiro Ishii, Takao Nakata

   Article

   Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

   Insert

   CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
   

   Use

   Tags

   CAAX and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-HOXD13-IDR-mCherry-CRY2-NLS

   Plasmid

   #145260

   Purpose

   Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13wt-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

   Plasmid

   #22034

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450M (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450M.Rac1 s…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-syn-FLEX-jYCaMP1

    Plasmid

    #135422

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Insert

    jYCaMP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid

    #174634

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-FLEX-jYCaMP1s

    Plasmid

    #135420

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cry2 mCherry GRK3 ct in pcDNA3.1

    Plasmid

    #64213

    Purpose

    mCherry fused between Cry2 and GRK3 ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK3 ct (GRK3 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

    Plasmid

    #22023

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450A.Rac1 s…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178827

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter;

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-CDreV

    Plasmid

    #140133

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CDreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA

    Plasmid

    #210503

    Purpose

    expresses LAUNCHERsbmv component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105982

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    FAPdL5-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin 1

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-FUSN-miRFP670-Cry2WT

    Plasmid

    #122441

    Purpose

    Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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