We narrowed to 166,418 results for: Gene

1. pUC19-Q03JI6

   Plasmid

   #103123

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
   

   Use

   CRISPR; Cloning vector

   Tags

   SV40NLS

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-GST-CHD5-L518A/Y619E-FRAG

   Plasmid

   #68874

   Purpose

   IPTG inducible bacterial expression of CHD5 fragment

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   331-672aa, L518A/Y619E

   Promoter

   tac

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-GST-CHD5-WT-FRAG

   Plasmid

   #68873

   Purpose

   IPTG inducible bacterial expression of CHD5 fragment

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   331-672aa

   Promoter

   tac

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pUCmini-iCAP-AAV.CAP-Mac

   Plasmid

   #200658

   Purpose

   Synthetic construct isolate AAV.CAP-Mac VP1 gene

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   Synthetic construct isolate AAV.CAP-Mac VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid insertion after Q588 of the AAV9 CAP…

   Promoter

   p41

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGR475

   Plasmid

   #213784

   Purpose

   Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

   Depositor

   Chang Liu

   Insert

   WT TP-DNAP1 (recoded)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-link-ymScarletI-SpHis5

   Plasmid

   #219844

   Purpose

   tagging vector to tag genes with ymScarletI and a HIS5 gene

   Depositor

   Bas Teusink

   Insert

   ymScarletI-SpHis5
   

   Tags

   ymScarletI

   Expression

   Yeast

   Available Since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET32a-Trx-His6-TEV_SacB

   Plasmid

   #164588

   Purpose

   Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

   Depositor

   Ernst Schonbrunn

   Insert

   TEV-SacB
   

   Tags

   Trx-His6 followed by TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK580

   Plasmid

   #71694

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTRY4_ Pi21

    Plasmid

    #126904

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAIP4_3OBS

    Plasmid

    #234227

    Purpose

    Heterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3OBS (TSG101 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCWU50

    Plasmid

    #232318

    Purpose

    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

    Depositor

    Chenggang Wu

    Insert

    Pfdx-E
    

    Expression

    Bacterial

    Promoter

    fdx

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti puro HA-H2BC11

    Plasmid

    #227588

    Purpose

    Lentiviral plasmid expressing human H2BC11 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    H2BC11 (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-C

    Plasmid

    #213496

    Purpose

    Encodes Node C of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    lambda cI 5G6GP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-zgc:152904

    Plasmid

    #192740

    Purpose

    Gateway entry vector encoding zebrafish zgc:152904

    Depositor

    Summer Thyme

    Insert

    zgc:152904 (zgc:152904 Zebrafish)
    

    Use

    Gateway entry vector

    Mutation

    N56D; 663T_664DinsA; 733V_772Edel (exon 24)

    Promoter

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-usp25

    Plasmid

    #192733

    Purpose

    Gateway entry vector encoding zebrafish usp25

    Depositor

    Summer Thyme

    Insert

    usp25 (usp25 Zebrafish)
    

    Use

    Gateway entry vector

    Mutation

    S403R; W622R; 725A_726AinsAA (rs508161559); A730T…

    Promoter

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-psmg1

    Plasmid

    #192723

    Purpose

    Gateway entry vector encoding zebrafish psmg1

    Depositor

    Summer Thyme

    Insert

    psmg1 (psmg1 Zebrafish)
    

    Use

    Gateway entry vector

    Mutation

    R96Q (rs514814252); T152M (rs501951686); T189A (r…

    Promoter

    None

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. thoc5.018.389

    Plasmid

    #137266

    Purpose

    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

    Depositor

    Tara Davis

    Insert

    thoc5 (THOC5 Human)
    

    Tags

    (His)6-TEV

    Expression

    Bacterial

    Mutation

    018-389

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge