We narrowed to 5,091 results for: codon optimized

1. pBad_crtEBI_64

   Plasmid

   #63809

   Purpose

   expresses crtEBI genes for neurosporene production

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   crtEBI operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   PBAD

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LacI_PlacO1_crtEBI_13

   Plasmid

   #63812

   Purpose

   expresses crtEBI genes for neurosporene production

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   crtEBI operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   Placo1

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBad_crtEBI_70

   Plasmid

   #63810

   Purpose

   expresses crtEBI genes for neurosporene production

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   crtEBI operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   PBAD

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP935

   Plasmid

   #159302

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRP1999

   Plasmid

   #159308

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP2000

   Plasmid

   #159309

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRP1975

   Plasmid

   #159310

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mNeongreen under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRP2002

   Plasmid

   #159311

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRP266

    Plasmid

    #159304

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mKate2 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRP265

    Plasmid

    #159301

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mTFP1 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRP1931

    Plasmid

    #159306

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mRuby2 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW003-Pc-mNeonGreen

    Plasmid

    #205018

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

    Plasmid

    #133801

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA4
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid

    #133802

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA5
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSW002-Pc-mNeonGreen

    Plasmid

    #205016

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

    Plasmid

    #134337

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

    Plasmid

    #134332

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Lmo
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

    Plasmid

    #134340

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Comments

    Promoter

    CaMKII

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge