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We narrowed to 611 results for: prime editing

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Showing: 521 - 560 of 611 results

  1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  6. lentiTet-LDLR-mCherryFus-In14-flanking BlastR

    Plasmid
    #254343
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, DNA from the 5' and 3' end of intron 14 inserted in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    In14 flanking insertion
    Available Since
    May 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    254343-plasmid-map-sequence-id-510276

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  7. lentiTet-LDLR-mCherryFus-In14-T713A3G-flanking BlastR

    Plasmid
    #254344
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, DNA from the 5' and 3' end of intron 14 inserted in LDLR, variant T713A3G introduced in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    In14 flanking insertion, T713A3G
    Available Since
    May 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    254344-plasmid-map-sequence-id-510240

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  8. lentiTet-LDLR-mCherryFus-In15-flanking-BlastR

    Plasmid
    #254345
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, DNA from the 5' and 3' end of intron 15 inserted in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    In15 flanking insertion
    Available Since
    May 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    254345-plasmid-map-sequence-id-510272

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  9. lentiTet-LDLR-mCherryFus-In15-Q770A3G-flanking-BlastR

    Plasmid
    #254346
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, DNA from the 5' and 3' end of intron 15 inserted in LDLR, variant Q770A3G introduced in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    In15 flanking insertion, Q770A3G
    Available Since
    May 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    254346-plasmid-map-sequence-id-510267

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  10. lentiTet-LDLR-mCherryFus-S213L-G219D-K223E-E101K BlastR

    Plasmid
    #254336
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variants S213L, G219D, K223E, E101K introduced in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    S213L, G219D, K223E, E101K
    Available Since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254336-plasmid-map-sequence-id-510290

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  11. lentiTet-LDLR-mCherryFus-S213L-G219D-K223E-E240K BlastR

    Plasmid
    #254338
    Purpose
    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variants S213L, G219D, K223E, E240K introduced in LDLR
    Depositor
    Richard Sherwood
    Article
    Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.
    Insert
    LDLR (LDLR Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    S213L, G219D, K223E, E240K
    Available Since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254338-plasmid-map-sequence-id-510262

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  12. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hPGK1
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  15. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185060-plasmid-map-sequence-id-375730

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  22. pMMACE DEST CMV H2B iRFP

    Plasmid
    #206253
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B iRFP713
    Use
    Recombinant baculovirus production, multimate/gat…
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206253-plasmid-map-sequence-id-406403

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  23. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206259-plasmid-map-sequence-id-406393

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  24. pMMDS DEST

    Plasmid
    #206264
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Type
    Empty backbone
    Use
    Cre-donor, multimate/gateway dest vector (multiba…
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206264-plasmid-map-sequence-id-406384

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  30. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  33. pMMK ENTR 3 CMV mito mCherry

    Plasmid
    #206256
    Purpose
    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mito mCherry
    Use
    Multimate/gateway entr 3
    Tags
    COX8 mitochondrial tag
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206256-plasmid-map-sequence-id-406392

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  34. pMMK ENTR 1 CMV mAG beta-catenin

    Plasmid
    #206258
    Purpose
    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mAG Ctnnb1 (Ctnnb1 Mouse)
    Use
    Multimate/gateway entr 1
    Tags
    monomeric Azami Green
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206258-plasmid-map-sequence-id-406391

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  35. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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Showing: 521 - 560 of 611 results