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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,308 results for: ENA

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Showing: 651 - 700 of 11308 results

  7. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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  10. pY2

    Plasmid
    #218115
    Purpose
    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.
    Depositor
    Carl Denard
    Article
    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218115-plasmid-map-sequence-id-431070

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  23. pHIs8-4b-SaDAH::G226D

    Plasmid
    #140130
    Purpose
    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli
    Depositor
    Jing-Ke Weng
    Article
    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:
    Insert
    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    changed Gly226 to Asp226
    Promoter
    T7
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140130-plasmid-map-sequence-id-275079

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  30. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110084-plasmid-map-sequence-id-211189

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  33. pConRef-2H12

    Plasmid
    #221182
    Purpose
    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221182-plasmid-map-sequence-id-437177

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  36. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  41. pMKR07

    Plasmid
    #240097
    Purpose
    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host
    Depositor
    Adrian Keatinge-Clay
    Article
    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ermE* promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240097-plasmid-map-sequence-id-478442

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  42. p2H12ref

    Plasmid
    #221188
    Purpose
    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen) and Ptet
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221188-plasmid-map-sequence-id-437182

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  46. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid
    #170316
    Purpose
    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.
    Depositor
    Andrew Carter
    Article
    Mali et al Science. 2021 Feb 26;371(6532):910-916.
    Insert
    Q22YU3 C-terminal truncation (Delta-C3)
    Tags
    Strep-Tag II
    Expression
    Insect
    Mutation
    Deletion of C-terminal C3-finger
    Promoter
    polyhedrin
    Available Since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170316-plasmid-map-sequence-id-333816

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  49. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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Showing: 651 - 700 of 11308 results