We narrowed to 4,214 results for: bre

1. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pb-miRE-tre-3xFLAG-hnRNPU

   Plasmid

   #196087

   Purpose

   Expresses mouse hnRNPU with a 3xFLAG tag in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAF-A (Hnrnpu Mouse)
   

   Use

   Tags

   N-terminal 3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CAGGS-safb∆sap-NLS-3XFLAG-V5

   Plasmid

   #196091

   Purpose

   Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 31-65 (SAP domain) of mouse S…

   Promoter

   CAGGS

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAGGS-safb∆rrm-NLS-3XFLAG-V5

   Plasmid

   #196093

   Purpose

   Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 428-506 (RRM) of mouse SAFB p…

   Promoter

   CAGGS

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMeCP2G pc1121

   Plasmid

   #167566

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Inserts

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBT-RP2.2

   Plasmid

   #189842

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBT-RP2.3

   Plasmid

   #189843

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBT-RP8.3

   Plasmid

   #189848

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   mRFP and tagBFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. A1-LCD-10R

   Plasmid

   #178664

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-10R (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   ten Arg residues removed

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A1-LCD+8D

    Plasmid

    #178674

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+8D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    eight Asp residues added

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A1-LCD+7R

    Plasmid

    #178667

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+7R (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Arg residues added

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. A1-LCD-2K

    Plasmid

    #178668

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-2K (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    two Lys residues removed

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. A1-LCD+4D

    Plasmid

    #178673

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+4D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    four Asp residues added

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. A1-LCD-6R

    Plasmid

    #178665

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-6R (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    six Arg residues removed

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. A1-LCD+2R

    Plasmid

    #178666

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+2R (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    two Arg residues added

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A1-LCD-6R+6K

    Plasmid

    #178670

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-6R+6K (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    six Arg residues replaced with Lys

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A1-LCD+12D

    Plasmid

    #178675

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+12D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twelve Asp residues added

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A1-LCD-3R+3K

    Plasmid

    #178669

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-3R+3K (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    three Arg residues replaced with Lys

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. A1-LCD+7F-7Y

    Plasmid

    #178660

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD +7F-7Y (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Tyr residues replaced with Phe

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A1-LCD+12E

    Plasmid

    #178676

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+12E (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twelve Glu residues added

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-Mm_MyoGEF(T575A)

    Plasmid

    #136328

    Purpose

    Mouse MyoGEF-T575A mutant in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    T575A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28B-PU.1-ETS-H3

    Plasmid

    #124435

    Purpose

    Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Residues 225 to 239 replaced with corresponding s…

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28B-PU.1-ETS-H3/S3

    Plasmid

    #124627

    Purpose

    Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 237 to 241 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28B-PU.1-ETS-S3

    Plasmid

    #124550

    Purpose

    Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 242 to 246 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28b-PU.1-ETS-H2

    Plasmid

    #123358

    Purpose

    Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 207 to 215 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid

    #79823

    Purpose

    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hGFAP-Cre

    Plasmid

    #40591

    Purpose

    Mouse astrocyte expression of Cre

    Depositor

    Albee Messing

    Article

    Zhuo et al Genesis. 2001 Oct;31(2):85-94.

    Insert

    Cre recombinase (GFAP Mouse, Phage, Human)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    MP-1 fragment (mouse protamine 1 intron and polya…

    Expression

    Mammalian

    Mutation

    Construct contains a nuclear-targeted Cre recombi…

    Promoter

    hGFAP promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. A1-LCD+7R+12D

    Plasmid

    #178678

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+7R+12D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Arg residues added, twelve Glu residues add…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLENTI_Hu_ECT2(WT)

    Plasmid

    #136330

    Purpose

    Lentiviral expression of human ECT2 WT

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    ECT2 (ECT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pb-miRE-tre-3xFLAG-hnRNPU∆RGG

    Plasmid

    #196088

    Purpose

    Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAF-A (Hnrnpu Mouse)
    

    Use

    Tags

    N-terminal 3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    removed last 154 amino acids on cterminal end

    Promoter

    TRE

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid

    #196092

    Purpose

    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 96-285 (predicted disordered …

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid

    #196094

    Purpose

    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 638-925 (predicted disordered…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid

    #196095

    Purpose

    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Contains only AA 619-925 (NLS and predicted disor…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. A1-LCD-2R-2K+3D

    Plasmid

    #178680

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-2R+2K+3D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    two Arg residues removed, two Lys added, three As…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. A1-LCD-9F+3Y

    Plasmid

    #178663

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-9F+3Y (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    nine Phe residues removed, three Tyr added

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. A1-LCD-14N+14Q

    Plasmid

    #178692

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-14N+14Q (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    fourteen Gln residues replaced with Asn

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. A1-LCD-10G+10S

    Plasmid

    #178682

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with ten Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-10G+10S (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    ten Gly residues replaced with Ser

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. A1-LCD+7K+12D

    Plasmid

    #178679

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+7K+12D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Lys residues added, twelve Glu residues add…

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. A1-LCD-30G+30S

    Plasmid

    #178684

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-30G+30S (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    thirty Gly residues replaced with Ser

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. A1-LCD+23G-23S

    Plasmid

    #178685

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+23G-23S (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty-three Ser residues replaced with Gly

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. A1-LCD-4R-2K+5D

    Plasmid

    #178681

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-4R+2K+5D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    four Arg residues removed, two Lys added, five As…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. A1-LCD-9F+6Y

    Plasmid

    #178661

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-9F+6Y (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    nine Phe residues removed, six Tyr added

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. A1-LCD-8F+4Y

    Plasmid

    #178662

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-8F+4Y (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    eight Phe residues removed, four Tyr added

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. A1-LCD+7R+10D

    Plasmid

    #178677

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+7R+10D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Arg residues added, ten Glu residues added

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. A1-LCD-12F+12Y

    Plasmid

    #178659

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD -12F+12Y (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twelve Phe residues replaced with Tyr

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. A1-LCD-23S+23T

    Plasmid

    #178693

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-23S+23T (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty-three Thr reisudes replaced with Ser

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. A1-LCD-10R+10K

    Plasmid

    #178671

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-10R+10K (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    ten Arg residues replaced with Lys

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. A1-LCD-20G+20S

    Plasmid

    #178683

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-20G+20S (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty Gly residues replaced with Ser

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge