Addgene

We narrowed to 1,953 results for: Endog

Showing: 261 - 280 of 1953 results

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pU1utr-EGFP

Plasmid

#140268

Purpose

U1A-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

U1A binding site

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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plet7d_stbC-EGFP

Plasmid

#140266

Purpose

LIN28-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Stabilized hairpin of miR-let7d

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDsRed-TSTEP_v2

Plasmid

#72336

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMos-3xP3DsRed-attP

Plasmid

#52904

Purpose

Mos1 transformation vector containing MCS, loxP, attP, 2 x 5HE

Depositor

Zach Adelman

Article

Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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TU6m-gRNAscaffHygroR

Plasmid

#165485

Purpose

S. pyogenes Cas9 guide RNA expression in tilapia cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Oreochromis mossambicus U6

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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EMM71T

Plasmid

#49044

Purpose

Backbone contains SHD/T 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

Depositor

Eric Mendenhall, Bradley Bernstein

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

Plasmid

#35193

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

tdtomato cyc loxP KAN loxP

Use

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pTSK241

Plasmid

#34550

Depositor

James McNally

Article

Karpova et al Science. 2008 Jan 25;319(5862):466-9.

Insert

Ace1 (CUP2 Budding Yeast)

Use

Integrative plasmid

Tags

GFP

Expression

Yeast

Mutation

Promoter

CAP2

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pAptamerCassette-EGFP

Plasmid

#140288

Purpose

Backbone for making protein-responsive mRNA switches

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMbd3EosHaloTAF_Hyg

Plasmid

#140958

Purpose

Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

methyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Expression

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-p65-AD

Plasmid

#138395

Purpose

RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-p65-AD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro

Plasmid

#227253

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-Puro

Plasmid

#207743

Purpose

Expression of the Cre recombinase linked to a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-2A-Puro

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Puro

Plasmid

#207727

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Reporter-1

Plasmid

#180218

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L

Plasmid

#209698

Purpose

Expresses MAC3-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Use

Tags

MAC3-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-dTAG-Zeo

Plasmid

#207738

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mStayGold-Zeo

Plasmid

#227255

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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