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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pCSCMV:tdTomato

Plasmid

#30530

Purpose

mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

Depositor

Gerhart Ryffel

Publication

Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

Insert

tdTomato

Expression

Mammalian

Mutation

R415H

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

EBFP2

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

Plasmid

#138147

Purpose

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Publication

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

GFP

Use

CRISPR and Lentiviral

Promoter

EFS (P2A)

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

127 Articles

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pCAGGS-mCherry

Plasmid

#41583

Depositor

Phil Sharp

Publication

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Publication

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Publication

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Expression

Worm

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

214 Articles

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pOGG004

Plasmid

#113979

Purpose

Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 1 golden gate cloning site

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

19 Articles

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pBV-Luc

Plasmid

#16539

Purpose

luciferase reporter plasmid with very low basal activity

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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