167,468 results

1. lenti SYN-FLAG-dCas9-VPR

   Plasmid

   #114196

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABEpuro-ERBB2

   Plasmid

   #40978

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-MHCK7-GCaMP6

   Plasmid

   #65042

   Purpose

   Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

   Depositor

   Nenad Bursac

   Article

   Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

   Insert

   GCaMP6
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Promoter

   MHCK7

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-FLAG-SZT2

   Plasmid

   #87034

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TBG-ER-TurboID

   Plasmid

   #149415

   Purpose

   Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   TBG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Expression

    Mammalian

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA GSK3 beta wt pcDNA3

    Plasmid

    #14753

    Purpose

    Mammalian expression of human GSK3 beta with HA tag

    Depositor

    Jim Woodgett

    Article

    He et al Nature. 1995 Apr 13. 374(6523):617-22.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA Myc DBC1

    Plasmid

    #35096

    Depositor

    Osamu Hiraike

    Article

    Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

    Insert

    deleted in breast cancer 1 (CCAR2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3XFlag-pA-Tn5-Fl

    Plasmid

    #124601

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Steven Henikoff

    Article

    Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

    Insert

    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
    

    Tags

    Protein A and Tn5 transposase fusion protein with…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TREtight-mTagBFP2-B19G (AAV1)

    Viral Prep

    #100799-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Promoter

    TREtight

    Tags

    BFP (in presence of Cre and 100798)

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-mito-Citron1

    Plasmid

    #134305

    Purpose

    mitochondria-targeted direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE47

    Plasmid

    #90385

    Purpose

    IFNB1 - Interferon beta gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    IFNB1

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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