We narrowed to 10,294 results for: otos

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Tol2 8xSTAR-sTomato-NLS. PGK-puro

Plasmid

#136261

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLS-SV40-mP-EGFP

Plasmid

#137724

Purpose

Lentivirus based EGFP expression vector

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Insert

SV40 enhancer

Use

Lentiviral

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2-WPRE

Plasmid

#218874

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

Type

Empty backbone

Tags

mNeonGreen11

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-STX17ΔNTD

Plasmid

#89941

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-161

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Clathrin LC-15

Plasmid

#55019

Purpose

Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin LC (CLTB Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mIRFP670nano3

Plasmid

#244915

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mIRFP670nano3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

ubiquitin C

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4 myc PGC-1 alpha

Plasmid

#10974

Purpose

Mammalian expression of PGC-1a with myc and his tags

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

PGC-1 alpha (PPARGC1A Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-calpainsensor

Plasmid

#36182

Purpose

detection of activity of ubiquitous calpains

Depositor

Isabelle Richard

Article

Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

Insert

alpha-Fodrin cleavage site (Sptan1 Mouse)

Tags

eCFP and eYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-CANTHipi

Plasmid

#53301

Purpose

Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtO

Use

Low copy number bacterial cloning vector

Tags

6His

Promoter

Trc

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGEN-dPspCas13b-NES-mCherry

Plasmid

#233609

Purpose

Expression of catalytically-inactive PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

dPspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Mutation

H133A/H1058A

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHLUM

Plasmid

#40276

Depositor

Markus Ralser

Article

Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

Inserts

LEU2 (LEU2 Budding Yeast)
URA3 (URA3 Budding Yeast)
MET15/17 (MET17 Budding Yeast)

Expression

Yeast

Promoter

endogenous LEU2, endogenous MET15/17, and endogen…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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sAB-K29

Plasmid

#204735

Purpose

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRSM32

Plasmid

#101634

Purpose

Encodes the fluorescent protein Rosmarinus for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Tags

6XHis

Expression

Bacterial

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CAND1

Plasmid

#20719

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

HA2

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Tags

mCherry

Expression

Worm

Promoter

Prig-3

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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