We narrowed to 9,797 results for: otos

1. pKLV-EF1amCherry-W

   Plasmid

   #159295

   Purpose

   Lentiviral vector expressing mCherry driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Sig10-Fc-IG

   Plasmid

   #183722

   Purpose

   Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

   Insert

   Siglec-10
   

   Use

   Retroviral

   Tags

   Fc of human IgG1

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Level 1 P1 OsActinP:Hygint:NosT

   Plasmid

   #165423

   Purpose

   GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
   

   Use

   Synthetic Biology; L1 p1(r) golden gate (moclo) h…

   Tags

   Expression

   Mutation

   Promoter

   Os Actin promoter :: Hygromycin resistance gene (…

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME V5-mTagBFP2 (JDW 830)

   Plasmid

   #224484

   Purpose

   Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS-H2B-mRFP1

   Plasmid

   #53745

   Purpose

   Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Limk1

   Plasmid

   #163414

   Purpose

   Expresses LIMK1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Limk1 (Limk1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-iGABASnFR2n-WPRE

   Plasmid

   #218875

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   Synapsin

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA2-CAND1

   Plasmid

   #20719

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pME EFS-rtTA/rtTA3 (JDW 1230)

   Plasmid

   #224525

   Purpose

   A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   EFS-rtTA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-hs_ICAM_short (JDW 1046)

    Plasmid

    #224480

    Purpose

    Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    min-ICAM2 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIB1-CreC(N1)

    Plasmid

    #75367

    Purpose

    Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Cre DNA recombinase, residues 106-343

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PA-Cdc42

    Plasmid

    #75263

    Purpose

    Light-activated Cdc42

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    Cell Division Cycle 42 (CDC42 Human)
    

    Use

    Tags

    6xHIs, LOV2, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    F56W

    Promoter

    CMV

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. BICDN-CIBN

    Plasmid

    #102253

    Purpose

    Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    BICDN-CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid

    #117656

    Purpose

    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Inserts

    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Bmp4

    Plasmid

    #163588

    Purpose

    Expresses BMP-4 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp4 (Bmp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP-GFP-FAPP(PHD)

    Plasmid

    #221748

    Purpose

    Stable expression of GFP-FAPP(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    FAPP (PLEKHA3 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–101

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Vimentin-C-18

    Plasmid

    #55157

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-AU1-mTOR-E2419K

    Plasmid

    #19994

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-E2419K (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Glutamine 2419 to Lysine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pMRX-IZ-ANXA5-mRuby3

    Plasmid

    #184891

    Purpose

    Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA5 (ANXA5 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. p5E TRE-3GV (JDW 1163)

    Plasmid

    #224476

    Purpose

    Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    TRE3GV promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Bmpr2

    Plasmid

    #163403

    Purpose

    Expresses BMPR-2 in mammalican cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CapChR1_pmCherry-N1

    Plasmid

    #188031

    Purpose

    Expresses CapChR1-mCherry in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMXs-Lhx3

    Plasmid

    #32927

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Lhx3 (Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEGFP-MyosinVb-Ctail

    Plasmid

    #110170

    Purpose

    Dominant negative effect by competing with MyosinVb C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1262-1670

    Promoter

    CMV

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28b_Q69L

    Plasmid

    #186272

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Use

    Tags

    His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed Q69 into L

    Promoter

    T7 promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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