-
Plasmid#227288PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.DepositorInsertsgRNA Targeting C-terminus of CEP192 (CEP192 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CEP135
Plasmid#227286PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.DepositorInsertsgRNA Targeting N-terminus of CEP135 (CEP135 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ARL13B
Plasmid#227276PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.DepositorInsertsgRNA Targeting C-terminus of ARL13B (ARL13B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM90
Plasmid#226682PurposeRpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)DepositorInsertsUseTagsMBP-HRVExpressionBacterialMutationS111TAGPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y
Plasmid#226349PurposeExpression of Titin V15P substrate with GS tailDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5
Plasmid#226352PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
Plasmid#226353PurposeExpression of titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper DGK alfa human
Plasmid#224574PurposeTo downregulate the expression of human DGK alfa in human cellsDepositorInsertsh RNAi Diacylglycerol kinase alfa human (DGKA Human)
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper DGK alfa mouse
Plasmid#224573PurposeNegative Control for expression downregulation of DGK alfa human in human cellsDepositorInsertsh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SOX17
Plasmid#222925PurposePiggyBac transposon plasmid for doxycycline inducible expression of SOX17DepositorInsertSOX17 (SOX17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mVprBP pc2953
Plasmid#201698PurposeExpresses of GFP-tagged VprBP in mammalian cellsDepositorInsertVprBP (Dcaf1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-mVprBP pc2954
Plasmid#201699PurposeExpresses of mcherry-tagged mVprBP in mammalian cellsDepositorInsertVprBP (Dcaf1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
T40A+Q54A
Plasmid#217913PurposeA plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutationsDepositorInsertPXP T40A+Q54A
UseTags6x His (N and C term)ExpressionBacterialMutationThreonine at position 41 and 253 mutated to Alani…PromoterT5Available sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE
Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN006A-P_V5_FLAG_C
Plasmid#218062PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN006A-P
UseLentiviralTagsV5-FLAGExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN001M-T_V5_FLAG_C
Plasmid#218063PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN001M-T
UseLentiviralTagsV5-FLAGExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN006A-P_V5_mNG_FLAG_C
Plasmid#218076PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN006A-P
UseLentiviralTagsFLAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001M-T
Plasmid#218078PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001M-T
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001T-N
Plasmid#218077PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001T-N
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN006A-P
Plasmid#218079PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN006A-P
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001K-M
Plasmid#218084PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001K-M
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN004M-4
Plasmid#218082PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN004M-4
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN008H-P
Plasmid#218081PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN008H-P
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN0059-C
Plasmid#218083PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN0059-C
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN006V-4
Plasmid#218080PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN006V-4
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)
Plasmid#208872PurposeFor expression in E. coli of NAP1 (delta-NDP52)DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationS37K/A44E (disrupts NDP52 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)
Plasmid#208873PurposeFor expression in E. coli of NAP1 (delta-TBK1)DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationL226Q/L233Q (disrupts TBK1 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)
Plasmid#208863PurposeStably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationS37K/A44E (disrupts NDP52 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)
Plasmid#208864PurposeStably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationI11S/L12S (disrupts FIP200 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)
Plasmid#208865PurposeStably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationL226Q/L233Q (disrupts TBK1 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Luciferase-CD4-bla
Plasmid#189981PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-mScarlet-I-CD4-bla
Plasmid#189984PurposeDonor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Luciferase-CD4-bla
Plasmid#189985PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Halo-CD4-bla
Plasmid#189986PurposeDonor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEWS
Plasmid#208799PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381A; S437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-3A
Plasmid#208800PurposeXenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationV473A; R476A; Y481APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only