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GFP-PIK3C2B

Plasmid

#161989

Purpose

Mammalian expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant, Volker Haucke

Publication

Marat et al Science. 2017 Jun 2;356(6341):968-972. d

Insert

PIK3C2B (PIK3C2B Human)

Tags

GFP

Expression

Mammalian

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Expression

Mammalian

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT-FLAG

Plasmid

#31385

Depositor

Yegor Vassetzky

Publication

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Type

Empty backbone

Tags

FLAG

Expression

Mammalian

Available since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EasyFusion mAID-mCherry with Neo

Plasmid

#113831

Purpose

EasyFusion vector for targeting endogeneous genes with mAID-mCherry

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mAID-mCherry

Use

Synthetic Biology

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSV40-mCherry-P2A-Hygro

Plasmid

#106478

Purpose

Expression of mCherry and Hygromycin resistance gene from SV40 promoter

Depositor

Alex Hewitt

Inserts

mCherry
Hygromycin B resistance

Expression

Mammalian and Plant

Promoter

SV40 promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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p404-MTS-mCherry-GFP1-10

Plasmid

#91958

Purpose

Expresses mCherry-GFP1-10 in yeast cell mitochondria

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

MTS-mCherry-GFP1-10

Expression

Yeast

Promoter

GAP

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-MAP4K1

Plasmid

#23484

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K1 (MAP4K1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHyg-AID*-9myc

Plasmid

#99518

Purpose

C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-BUB1

Plasmid

#23612

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1 (BUB1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Publication

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-EXOSC10

Plasmid

#23674

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EXOSC10 (EXOSC10 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLX304 Flag-mCherry-eEF1A1_IRES-GFP

Plasmid

#198383

Purpose

eEF1A1 fluorescent reporter

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eEF1A1 (EEF1A1 Human)

Use

Lentiviral

Tags

Flag-mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Publication

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Human, Synthetic)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ER_K0

Plasmid

#133863

Purpose

Expression of ER-targeted Ribosome non-stalling reporter

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

ER_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAG31

Plasmid

#35124

Depositor

John McCusker

Publication

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

patMX3

Use

Yeast replicating

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-Spike

Plasmid

#173921

Purpose

Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

Depositor

Andreas Stuermer

Insert

SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)

Tags

no tags, native protein

Expression

Bacterial and Mammalian

Promoter

standard CMV

Available since

April 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pKK-TEV-BirA-FLAG

Plasmid

#105786

Purpose

Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-BirA-FLAG

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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