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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,402 results for: 221

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Showing: 7101 - 7150 of 8402 results

  3. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

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  4. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

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  6. NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

    Plasmid
    #236863
    Purpose
    Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236863-plasmid-map-sequence-id-493429

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  7. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

    Plasmid
    #236854
    Purpose
    Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61K
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236854-plasmid-map-sequence-id-477412

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  14. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  15. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  29. pLY237

    Plasmid
    #184150
    Purpose
    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-R1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    araBAD promoter
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184150-plasmid-map-sequence-id-376095

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  30. pLY251

    Plasmid
    #184153
    Purpose
    Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-Ar2
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    araBAD promoter
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184153-plasmid-map-sequence-id-376090

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  32. pNHT7

    Plasmid
    #186753
    Purpose
    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Type
    Empty backbone
    Use
    Synthetic Biology; Cloning backbone
    Promoter
    T7
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186753-plasmid-map-sequence-id-375095

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  33. pSLiP-G2

    Plasmid
    #188964
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188964-plasmid-map-sequence-id-371924

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  44. pYPQ265H

    Plasmid
    #149554
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    hAID-ziSpyMacCas9(D10A)-UGI
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149554-plasmid-map-sequence-id-308763

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  45. pYPQ266B

    Plasmid
    #149555
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ziSpyMacCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149555-plasmid-map-sequence-id-306140

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  47. pLY151

    Plasmid
    #130947
    Purpose
    pspFΔHTH::λN22plus gene controlled by promoter PrhaB
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    rhaS
    pspFΔHTH::λN22plus
    Use
    Synthetic Biology
    Tags
    pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    The HTH domain of the pspF gene was deleted (297-…
    Promoter
    Pcon and PrhaB
    Available Since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    130947-plasmid-map-sequence-id-258929

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Showing: 7101 - 7150 of 8402 results