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We narrowed to 15,163 results for: GRN

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Showing: 7101 - 7150 of 15163 results

  7. tRNA-gRNA position [M1_2] (GB1209)

    Plasmid
    #75410
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_2]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75410-plasmid-map-sequence-id-146027

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  9. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  10. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    U6 and synthetic Probasin ARRx2
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

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  11. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  12. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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  13. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207357-plasmid-map-sequence-id-408418

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  19. (pRZ60) AAV: U6-gRNA(cs1)-EF1a-eGFP

    Plasmid
    #254582
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and capture sequence in scaffold and eGFP from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254582-plasmid-map-sequence-id-510213

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  20. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP

    Plasmid
    #255027
    Purpose
    Lentiviral plasmid designed to express a SaCas9 compatible gRNA expression cassette and dSaCas9-KRAB-T2A-GFP protein; Similar to Plasmid #162334, but with GFP instead of Puro
    Depositor
    Jonathan Ploski
    Insert
    dSaCas9
    Use
    CRISPR and Lentiviral
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255027-plasmid-map-sequence-id-509438

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  21. pLV U6 sgRNA2.0 pp7 EFS Puro 2A.BFP

    Plasmid
    #255463
    Purpose
    Expresses Puro and BFP and has an sgRNA2.0 scaffold for insertion of CRISPR activation gRNAs
    Depositor
    Le Cong
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    255463-plasmid-map-sequence-id-508841

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  24. (pRZ318) AAV: U6-gRNA(FLEX)-EF1a-mScarlet

    Plasmid
    #254587
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and 10x FLEX preferred scaffold and mScarlet from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254587-plasmid-map-sequence-id-505743

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  25. (pRZ320) AAV: U6-gRNA(FLEX)-EF1a-ZsGreen

    Plasmid
    #254588
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and 10x FLEX preferred scaffold and ZsGreen from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254588-plasmid-map-sequence-id-505741

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  26. (pRZ76) AAV: U6-gRNA(cs1)-EF1a-mCherry

    Plasmid
    #254583
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and capture sequence in scaffold and mCherry from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254583-plasmid-map-sequence-id-505738

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  28. pYJ30-lenti-U6-MAFA-acti-gRNA1-MS2

    Plasmid
    #131073
    Purpose
    Activation of Mafa via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA1 (MAFA Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131073-plasmid-map-sequence-id-267717

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  29. pYJ31-lenti-U6-MAFA-acti-gRNA2-MS2

    Plasmid
    #131074
    Purpose
    Activation of Mafa via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA2 (MAFA Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131074-plasmid-map-sequence-id-256658

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  30. pYJ32-lenti-U6-MAFA-acti-gRNA3-MS2

    Plasmid
    #131075
    Purpose
    Activation of Mafa via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA3 (MAFA Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131075-plasmid-map-sequence-id-267719

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  35. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid
    #240865
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240865-plasmid-map-sequence-id-482051

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  36. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid
    #240094
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240094-plasmid-map-sequence-id-482052

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  37. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid
    #241026
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven empty sgRNA
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241026-plasmid-map-sequence-id-482053

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  38. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  39. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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Showing: 7101 - 7150 of 15163 results