We narrowed to 3,074 results for: eed

1. pCRISPR2_IHF_Opt

   Plasmid

   #149395

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is optimized at key positions needed…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB101

   Plasmid

   #169110

   Purpose

   Feedback-controlled AraC circuit

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   araC (araC Escherichia coli K12 MG1655)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRBad-Z-C-DiB-RM

   Plasmid

   #168477

   Purpose

   C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   Leucine Zipper + C-fragment of DiB-RM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-Z-N-DiB-RM

   Plasmid

   #168475

   Purpose

   N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   N-fragment of DiB-RM + Leucine Zipper
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRWJB006

   Plasmid

   #160113

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRWJB004

   Plasmid

   #160112

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   UBQ10

   Available since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVH009

   Plasmid

   #80399

   Purpose

   Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   3xFLAG-Venus YFP-AAV
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal LZx domain and N-terminal 3xFLAG tag, …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPAt2S3 (GB0029)

   Plasmid

   #68162

   Purpose

   Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   At2S3 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB-TRE-CRISPRoff-EF1A-TetOn

    Plasmid

    #203355

    Purpose

    Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    CRISPRoff-TetOn
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPPED

    Plasmid

    #162468

    Purpose

    Destination Expression vector for dicot plant prime editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine based sequence o…

    Promoter

    2x35S

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG225 HIPK1 3'UTR mut

    Plasmid

    #12044

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    HIPK1 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAG177 HIPK1 3'UTR mut

    Plasmid

    #12035

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N10 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    UBQ10

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLuc-CDS-m215-2

    Plasmid

    #42102

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Silent mutations were introduced in the seed-comp…

    Promoter

    SV40

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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