We narrowed to 43,183 results for: cha
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Plasmid#211334PurposeVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseAAVTagsStayGoldExpressionMutationChanged Trp-795 to ArgPromoterEF1aAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1(W795R)-TwinStrep-His
Plasmid#210817PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseTagsTwinStrep, 10xHisExpressionMammalianMutationChanged Trp-795 to ArgPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr_LF(C199S)
Plasmid#216114PurposeExpresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertoROS-Gr_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-WM
Plasmid#208798PurposeXenopus laevis His6-Myc-DONSON (W234L, M463T)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationW234L; M463TPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-ctfR1
Plasmid#207992PurposeCRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertctfR1
UseCRISPRTagsExpressionMutationPromoterAspergillus flavus U6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control V5-Twist
Plasmid#201376PurposeExpresses Twist for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R1R2
Plasmid#191440PurposeExpresses the human TLN1 R1R2 domains in bacteriaDepositorInsertHuman Talin1 R1R2 domains (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_B1ChR2_TS_mScarlet_ER
Plasmid#195191PurposeHighly K+ selective channelrhodopsinDepositorInsertBilabrum sp. K+ selective channelrhodopsin 2
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-HA-BioID2-SAPAP3
Plasmid#176858PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in astrocytesDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1 with cleavable His tag
Plasmid#181813PurposeExpresses human MUC2 D1 with cleavable His tag, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-MR1 (M50P)
Plasmid#178651PurposeMammalian expression of myc-tagged MR1 (M50P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationM50PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 MR1 (H83P)
Plasmid#178652PurposeMammalian expression of myc-tagged MR1 (H83P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationH83PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 MR1 (Q153P)
Plasmid#178653PurposeMammalian expression of myc-tagged MR1 (Q153P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationQ153PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_ShDHN (pBS0797)
Plasmid#185260PurposeFor the mammalian expression of the tomato plant protein DHN_ShDHN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_ShDHN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-MYH10_sgRNA
Plasmid#183887PurposepX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.DepositorInsertMYH10 sgRNA spacer (MYH10 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-MYH10_sgRNA
Plasmid#183886PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.DepositorInsertMYH10 sgRNA spacer (MYH10 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_COX4
Plasmid#177983Purposelentiviral vector expressing Cas9 and a sgRNA targeting COX4DepositorInsertsgRNA targeting COX4
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
L2_NOP1gRNA-Cas9-CsA
Plasmid#136139PurposePlasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorUseTags6xHisExpressionYeastMutationK509APromoterGAL1Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PsuACR_433-EYFP
Plasmid#103529PurposeAnion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin PsuACR_433
UseTagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1613
Plasmid#63789PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection markerDepositorInserttDimer2-Strep-tag II followed by HIS5
UseE. coli cloning vectorTagstDimer2-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1122
Plasmid#63790PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection markerDepositorInserteSapphire-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseSapphire-Twin-Strep tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2deltaC
Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS134-Pleum
Plasmid#60339PurposeContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(936-1113)
Plasmid#35075DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 936-1113PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1113-1316)
Plasmid#35077DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1113-1316PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(894-1057)
Plasmid#35074DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 894-1057PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
CS2 luc MutA
Plasmid#13498DepositorInsertFstl1 (Fstl1 Mouse)
UseLuciferaseTagsExpressionMutationcontains 3 tandem copies of the following sequenc…PromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
CS2 luc MutB
Plasmid#13499DepositorInsertFstl1 (Fstl1 Mouse)
UseLuciferaseTagsExpressionMutationcontains 3 tandem copies of the following sequenc…PromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVSp1 pET24b
Plasmid#128088PurposeBacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel proteinDepositorInsertNaVSp1p
UseTags3C Cleavage, His Tag, and MBPExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralTagsExpressionMutationWTPromoterU6Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLX2-hygro
Plasmid#97330PurposeGenerating lentiviruses to express Dlx2 and hygromycin resistanceDepositorInsertDLX2 (Dlx2 Mouse)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-Ascl1-puro
Plasmid#97329PurposeGenerating lentiviruses to express Ascl1 and puromycin resistanceDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTET-ONAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only