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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,277 results for: Met;

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Showing: 2861 - 2880 of 19277 results

  3. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  10. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  15. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

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  20. PB-CT3G-CERP2

    Plasmid
    #175499
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175499-plasmid-map-sequence-id-345877

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Showing: 2861 - 2880 of 19277 results