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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Tags

MiniR

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Entry vector

Tags

His and V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Expression

Mammalian

Promoter

SFFV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-XRCC6

Plasmid

#192302

Purpose

Gateway entry vector for an inducible 3xFLAG-XRCC6

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

XRCC6 (XRCC6 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2

Plasmid

#67678

Purpose

Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L3-NF-L1-dMb2Cas12a

Plasmid

#177686

Purpose

Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

2xFokI-dMb2Cas12a

Use

CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pR-mClover3

Plasmid

#228605

Purpose

Mammalian expression of mClover3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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MAC-N-GFP

Plasmid

#172974

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Tags

MAC-tag

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Multisite gateway entry clone

Promoter

CAG Promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TTGmiRc2

Plasmid

#25753

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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