We narrowed to 6,478 results for: nls
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pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
TagsNuclear localization signal (NLS) and mCherryExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
TagsSV40 NLSExpressionPlantPromoter35SAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas5c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas8
Plasmid#178880PurposeExpression of human codon-optimized NlaCas8 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas8c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHW00019
Plasmid#222448PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.DepositorInsertGAL4AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4459 FLAG-hPLIC-1 NTF
Plasmid#8664DepositorInserthPLIC-1 (UBQLN1 Human)
TagsFLAGExpressionMammalianMutationN-terminal fragment: amino acids 1-112 of hPLIC-1…Available SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCHW00016
Plasmid#222447PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.DepositorInsertp65AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA2a
Plasmid#47762Purposemammalian expression of rat PMCA2aDepositorAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only
