We narrowed to 11,401 results for: NSI

1. hnRNPAB X2-RBD

   Plasmid

   #216516

   Purpose

   Expresses MBP-tagged RNA binding domain of hnRNPAB X2

   Depositor

   Nicolas Fawzi

   Article

   O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

   Insert

   hnRNPAB X2-RBD
   

   Use

   Tags

   6xHis tags-MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 2-64 and 226-304

   Promoter

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJL CusS NarX Chimera

   Plasmid

   #225652

   Purpose

   Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   CusS-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJL NrsS NarX Chimera

   Plasmid

   #225653

   Purpose

   Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NrsS-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJL RssA NarX Chimera

   Plasmid

   #225654

   Purpose

   Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   RssA-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJL VanS NarX Chimera

   Plasmid

   #225655

   Purpose

   Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   VanS-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMART-HCKan-yibDp-roGFP2

   Plasmid

   #202462

   Purpose

   Expresses redox sensitive GFP (roGFP) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. iNicSnFR12

   Plasmid

   #227142

   Purpose

   Genetically encoded fluorescent biosensor for nicotine detection

   Depositor

   Henry Lester

   Article

   Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Insert

   iNicSnFR12
   

   Use

   Tags

   6xHis-HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSF_ApyA

   Plasmid

   #226249

   Purpose

   Production of unmodified ApyA peptide.

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Insert

   His6_ApyA
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB65

   Plasmid

   #226319

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis- (VTG to AAA MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAMC042z21

    Plasmid

    #209747

    Purpose

    RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    sfGFP - tRNA operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE5423_p3WJdB-glcO60

    Plasmid

    #221194

    Purpose

    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO60-3WJdB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter with downstream glcO60 operator

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-PML RING

    Plasmid

    #185631

    Purpose

    expression of GST-tagged PML RING domain in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-PD-L1-SNAP

    Plasmid

    #223577

    Purpose

    Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-CLIP-PD-L1

    Plasmid

    #223571

    Purpose

    Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET-Cm-CspA1

    Plasmid

    #215398

    Purpose

    Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA1
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli BL21(DE3)

    Promoter

    T7 promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7-LacO-Halo-PILATeS-RGD (200nM)

    Plasmid

    #216716

    Purpose

    Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-PILATeS-RGD
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

    Plasmid

    #216717

    Purpose

    Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-RGD-PILATeS
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7-LacO-Halo-PILATeS-RGD (700pM)

    Plasmid

    #216714

    Purpose

    Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-PILATeS-RGD
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

    Plasmid

    #216715

    Purpose

    Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-RGD-PILATeS
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BoxB-MS2 adRNA (RAB7A-GAC site)

    Plasmid

    #170149

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(GAC)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MS2-adRNA (RAB7A-GAC site)

    Plasmid

    #170140

    Purpose

    AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-RAB7A(GAC)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lneg-INTR-GFP

    Plasmid

    #208902

    Purpose

    encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

    Insert

    INTR
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28_human-pro-IL-18_E192K_D193K

    Plasmid

    #214322

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    E192K_D193K

    Promoter

    T7

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28_human-pro-IL-18_I48N

    Plasmid

    #214323

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    I48N

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMS110

    Plasmid

    #215674

    Purpose

    Split hygromycinR landing pad insertion plasmid for ChrIII

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    3' ∆HYGR is promoterless and encodes aa60-341

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYSD101

    Plasmid

    #212925

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

    Plasmid

    #214339

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ENGD29DDEN_N36Y_H41E

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_human-pro-IL-18_LES33AAA

    Plasmid

    #214326

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    LES33AAA

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28_human-pro-IL-18_DDEN29ENGD

    Plasmid

    #214325

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    DDEN29ENGD

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_human-pro-IL-18_V47N_I48N

    Plasmid

    #214324

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    V47N_I48N

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a_Pro-IL-18_rat

    Plasmid

    #214333

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Rat)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYSD140

    Plasmid

    #212927

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYSD113

    Plasmid

    #212926

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYSD100

    Plasmid

    #212924

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGG-mTP-TurboID

    Plasmid

    #209396

    Purpose

    Transiently expressing TurboID fused with a Mitochondrial transit peptide (mTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    mTP-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204797

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pENTR2b-mScarlet

    Plasmid

    #207961

    Purpose

    Gateway entry vector with the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mScarlet
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mt deltaN20 TFB2MdeltaC7

    Plasmid

    #205060

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 7 aa

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mt deltaN20 TFB2MdeltaC13

    Plasmid

    #205063

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 13 aa

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge