We narrowed to 41,324 results for: REP

1. pETM6-E12-vioABECD

   Plasmid

   #66537

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAP2

   Plasmid

   #73211

   Purpose

   express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   AAP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT3GEPIR

   Plasmid

   #111177

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRubiG-T2A-Cas9

   Plasmid

   #75348

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-mPKR-FLAG-IG

   Plasmid

   #101792

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse PKR

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Pkr (Eif2ak2 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-4031NES

    Plasmid

    #105241

    Purpose

    A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    AMPK-EV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. -5.5kb-UCP1-GFP

    Plasmid

    #104585

    Purpose

    Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

    Depositor

    Irfan Lodhi

    Article

    Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

    Insert

    UCP1 promoter (Ucp1 Mouse)
    

    Use

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UCP1

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid

    #66128

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -1000 to -1

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Tags

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. SuperNova/pRSETB

    Plasmid

    #53234

    Purpose

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

    Depositor

    Takeharu Nagai

    Article

    Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

    Insert

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

    Plasmid

    #86828

    Purpose

    Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_Sec61B-V5-APEX2

    Plasmid

    #83411

    Purpose

    Expressed C-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    Sec61B-V5-APEX2 (SEC61B Human, Synthetic)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. SGEN

    Plasmid

    #111171

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    SFFV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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