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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,744 results for: Spr

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Showing: 2881 - 2900 of 25744 results

  2. pSBY3_ligD-HygR

    Plasmid
    #104625
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104625-plasmid-map-sequence-id-201573

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  4. pYPQAT45

    Plasmid
    #223417
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223417-plasmid-map-sequence-id-453515

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  5. pJL31

    Plasmid
    #227428
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    mWasabi
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227428-plasmid-map-sequence-id-455967

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  6. pJL32

    Plasmid
    #227429
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    tdTomato
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227429-plasmid-map-sequence-id-455968

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  12. pYPQAT60

    Plasmid
    #223432
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223432-plasmid-map-sequence-id-475539

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  13. pYPQAT61

    Plasmid
    #223433
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223433-plasmid-map-sequence-id-454248

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  15. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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Showing: 2881 - 2900 of 25744 results