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pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pTH729-minRLuc

Plasmid

#38225

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Mutation

Every codon of the Renilla coding sequence was ex…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH738-CENBEVYslow

Plasmid

#38220

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

GCN4-based single uORF-containing 5'-UTR

Expression

Yeast

Mutation

The start codons of uORFs2, 3 and4 of the yeast G…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHL1324

Plasmid

#37638

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pCon and pConNoHind

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
PCDNA3 T7 DLC2

Plasmid

#24268

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (3SA)

Plasmid

#23100

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 55/65/73 to alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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HSF1-GFPN3

Plasmid

#32538

Depositor

Stuart Calderwood

Article

Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

Insert

HSF1 (HSF1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-VPS35-mScarlet

Plasmid

#215474

Purpose

Expression vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-Paxillin-EGFP

Plasmid

#215530

Purpose

Lentiviral transfer vector with EGFP-tagged paxillin

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PXN (PXN Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pXD70LacZ6-LacI-Ptac(LacO)-LacZ

Plasmid

#191621

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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