We narrowed to 13,030 results for: HAL

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MTK234_028

Plasmid

#123908

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hThal5.10-2_hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_029

Plasmid

#123909

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hThal5.10-2_hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 C539A

Plasmid

#133008

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

C539A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 H524A

Plasmid

#133007

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

H524A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 D353A

Plasmid

#133006

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

D353A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJB557

Plasmid

#115967

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJB554

Plasmid

#115964

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW101

Plasmid

#115944

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW098

Plasmid

#115941

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW097

Plasmid

#115940

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW095

Plasmid

#115938

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW084

Plasmid

#115932

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/15 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[Tra].1026G

Plasmid

#112689

Purpose

express gRNA targeting Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJB553

Plasmid

#115963

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 1xTetApt-STOP

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJB555

Plasmid

#115965

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW096

Plasmid

#115939

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP 2x K-turn

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW100

Plasmid

#115943

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/15 SGP 2x K-turn

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#115929

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW082

Plasmid

#115930

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW083

Plasmid

#115931

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/14 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0109

Plasmid

#117571

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thaliana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62590} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0098

Plasmid

#117570

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thaliana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62600} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Synthetic, Mustard Weed)

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.364

Plasmid

#105572

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MYB-TAD(251-327)

Plasmid

#105619

Purpose

bacterially express GST tagged MYB TAD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB-TAD domain (Myb Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 251-327

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-TAF12-HFD(50-130)

Plasmid

#105615

Purpose

transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LENC-shTAF12.364

Plasmid

#105579

Purpose

retrovirally express TAF12 shRNA with Neo resistance and mCherry marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mus-Bnip3_deltaExon3

Plasmid

#100793

Purpose

Mammalian expression of Bnip3_deltaExon3 splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mouse-Bnip3_deltaExon3 (Bnip3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Exon3 deletion

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1::CDKA;1:YFP

Plasmid

#80685

Purpose

binary Expression vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

Insert

CDKA;1 (CDC2 Mustard Weed)

Tags

YFP

Expression

Plant

Promoter

ProCDKA;1

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRS315-PGK-SEIPIN2

Plasmid

#96987

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Expression

Yeast

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN3N-1C

Plasmid

#96983

Purpose

Express mutated Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 507 nucleotides from the N terminus…

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN1N-3C

Plasmid

#96982

Purpose

Express mutated Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 108 nucleotides from the N terminus…

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA27_no stop

Plasmid

#83860

Purpose

Gateway donor vector for IAA27 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA27 (PAP2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA8_no stop

Plasmid

#83874

Purpose

Gateway donor vector for IAA8 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA5_no stop

Plasmid

#83868

Purpose

Gateway donor vector for IAA5 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA5 (IAA5 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA3_no stop

Plasmid

#83863

Purpose

Gateway donor vector for IAA3 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA28_no stop

Plasmid

#83861

Purpose

Gateway donor vector for IAA28 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_568478.1 (IAA28 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA15_no stop

Plasmid

#83853

Purpose

Gateway donor vector for IAA15 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK381

Plasmid

#71573

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Expression

Bacterial

Mutation

changes aspartate-317 to glycine

Promoter

recA

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK382

Plasmid

#71574

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Expression

Bacterial

Mutation

changes aspartate-317 to asparagine; silent mutat…

Promoter

recA

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFFV-Cx45

Plasmid

#69808

Purpose

mammalian expression of chick Cx45

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1992 Nov;71(5):1277-83.

Insert

Cx45 (GJC1 Chicken)

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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