We narrowed to 59,078 results for: %s

1. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-HOXD13-IDR-mCherry-CRY2-NLS

   Plasmid

   #145260

   Purpose

   Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13wt-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-45b-HOXD13(+10A)-IDR-mCherry

   Plasmid

   #145282

   Purpose

   Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+10A)-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLEX301-ARF6-T157N-TagRFP

   Plasmid

   #162030

   Purpose

   Fast cycling ARF mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T151N

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX335A_hCas9(D10A)_IRF8gRNA2

   Plasmid

   #89720

   Purpose

   Knockout IRF8 in human cells

   Depositor

   Martin Zenke

   Article

   Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

   Insert

   IRF8 gRNA2 (IRF8 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBactin-Akna-mEGFP

   Plasmid

   #196866

   Purpose

   Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mEGFP (Akna Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. iBE3 CLYBL

   Plasmid

   #174569

   Purpose

   doxycycline inducible BE3 expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet ON

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1M-Tm-antimCherry

   Plasmid

   #206952

   Purpose

   Mammalian expression of 1M-Tm EMcapsulins targeting mCherry

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   MmMT3, TmEnc, antimCherry intrabody (LaM-4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLEX307-SARS-CoV-3CL-C145A

    Plasmid

    #160281

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX304-ARF5-mNeonGreen

    Plasmid

    #162025

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX307-Bat-CoV-HKU9-3CL-C144A

    Plasmid

    #160285

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX307-MERS-CoV-3CL-C148A

    Plasmid

    #160283

    Purpose

    Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C148A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145263

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+9A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307-IBV-3CL-C143A

    Plasmid

    #160289

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C143A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-Enhancer-ZC3H12D_PromoterMUT

    Plasmid

    #153068

    Purpose

    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

    Promoter

    None

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-Basic-Fascin-Promoter 402 mutation

    Plasmid

    #89825

    Purpose

    Fascin promoter for luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -370 Smad binding element mutated from CAGAC to T…

    Promoter

    SV40

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sfGFP-SasG(3G)-mCherry

    Plasmid

    #206945

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (3G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (3G)-mCherry
    

    Use

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sfGFP-SasG(7G)-mCherry

    Plasmid

    #206949

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (7G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (7G)-mCherry
    

    Use

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge