We narrowed to 14,510 results for: TIM

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Plasmid

#111160

Purpose

miR-E (miR-30 variant)-based RNAi with GFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-mCherry-XL (R125K)

Plasmid

#188054

Purpose

Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

6xHis

Expression

Bacterial

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

araBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163072

Purpose

Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry_gRNA

Plasmid

#80457

Purpose

gRNA expression vector with mCherry transfection control

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Inserts

sp Cas9 gRNA
mCherry

Use

CRISPR

Expression

Mammalian

Promoter

U6 and chicken β-actin promoter

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-mCh-bla

Plasmid

#210260

Purpose

Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
mCherry

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

Plasmid

#63166

Purpose

GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-HA"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CAMKIIbeta(T287D)

Plasmid

#21223

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CAMKIIbeta(T287D) Lab plasmid CF 0001.

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Gateway entry vector

Tags

mCherry

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEB2-TagRFP-T

Plasmid

#103998

Purpose

Plasmid encoding TagRFP-T

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP-T

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP35_MARV

Plasmid

#135471

Purpose

Encodes Marburg virus VP35 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

Insert

VP35

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSTAR-mOrange2-sfGFP

Plasmid

#115693

Purpose

Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

Depositor

David Suter

Article

Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

Insert

SA-mOrange2-sfGFP-SD

Use

Lentiviral

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCytERM-mTagBFP2

Plasmid

#184940

Purpose

ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-mTagBFP2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPIDCB2

Plasmid

#169900

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Dec. 24, 2021

Availability

Academic Institutions and Nonprofits only

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R57C10-Flp2

Plasmid

#63172

Purpose

R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

yeast wild type containing a Glycine at amino acid 5 of the protein sequence

Expression

Insect

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only