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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,304 results for: TIM;

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Showing: 2921 - 2940 of 14304 results

  3. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  7. ShuttleNSE

    Plasmid
    #50958
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to pShuttle-CMV (Addgene #16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Promoter
    NSE
    Available Since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50958-plasmid-map-sequence-id-76714

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  8. FCK-Halo-GFP

    Plasmid
    #14750
    Purpose
    Lentiviral expression of Halo-GFP for neuronal inhibition
    Depositor
    Edward Boyden
    Article
    Han et al PLoS One. 2007 Mar 21;2(3):e299.
    Insert
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N. pharaonis halorhodopsin is mammalian codon-opt…
    Promoter
    CaMKIIa
    Available Since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14750-plasmid-map-sequence-id-192940

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  12. pYSD085

    Plasmid
    #212922
    Purpose
    Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    649 Stalk
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212922-plasmid-map-sequence-id-421794

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  15. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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Showing: 2921 - 2940 of 14304 results