We narrowed to 43,229 results for: cha

1. pUDR211

   Plasmid

   #113870

   Purpose

   expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA3-HXT8 / sgRNA4-HXT14
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUDR418

   Plasmid

   #113875

   Purpose

   expression of double Cas9 programming sgRNA11 targetting STL1

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   doble sgRNA11-STL1
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSP64-hERG1a F656A

   Plasmid

   #53054

   Purpose

   Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Phenylalanine 656 to Alanine

   Promoter

   SP6

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSP64-hERG1a Y652A

   Plasmid

   #53053

   Purpose

   Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Tyrosine 652 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-BE3-PGK-Puro

   Plasmid

   #110842

   Purpose

   Lentiviral vector for constitutive expression of BE3 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-BE3-P2A-Puro

   Plasmid

   #110838

   Purpose

   Lentiviral vector for constitutive expression of BE3 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-BE3-FNLS

   Plasmid

   #110836

   Purpose

   CMV expression vector for BE3-FNLS construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mouse c-myc (exons II and III)

   Plasmid

   #8448

   Depositor

   Frederic Mushinski

   Article

   Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

   Insert

   Mouse c-myc (exons II and III) (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Exons II and III only

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

9. BRCA1-(170-360)

   Plasmid

   #35066

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 170-360

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MZB150

    Plasmid

    #217840

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    ARNO (CYTH2 Human)
    

    Use

    Tags

    10x His and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Deleted residues 1-51 and 253-400

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDA355_sgCiPOLR2D

    Plasmid

    #229022

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    POLR2D gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRDA355_sgCiPELO #2

    Plasmid

    #229020

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting PELO

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXPR_051d_sgCiChr2-2

    Plasmid

    #228940

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

    Plasmid

    #230935

    Purpose

    gRNA cloning vector encoding BFP and puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SFFV-CALU-WPRE-UbC-mCherry

    Plasmid

    #225952

    Purpose

    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Bart Nieuwenhuis, Richard Eva

    Inserts

    CALU (CALU Human)
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid

    #228400

    Purpose

    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    NES/PKI-mNeonGreen2x-NLS/FUS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

    Plasmid

    #228398

    Purpose

    Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    NLS/cMyc-AcGFP2x-NES/iKB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

    Plasmid

    #228399

    Purpose

    Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    3xFLAG-NLS/SV40-AcGFP-NES/PKI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB8709

    Plasmid

    #175224

    Purpose

    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL092W (CEN.PK strain)
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT-C34-L

    Plasmid

    #223967

    Purpose

    Anti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid

    #216112

    Purpose

    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dyn1 R271E D291R

    Plasmid

    #198342

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dyn1 R271E

    Plasmid

    #198343

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dyn1 R271E E720R

    Plasmid

    #198344

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Dyn1 K44A

    Plasmid

    #198340

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Dyn1 D406A

    Plasmid

    #198341

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid

    #216116

    Purpose

    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-DONSON-DEWS

    Plasmid

    #208799

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A; S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-DONSON-3A

    Plasmid

    #208800

    Purpose

    Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    V473A; R476A; Y481A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-DONSON-Δ24

    Plasmid

    #208801

    Purpose

    Xenopus laevis His6-Myc-DONSON (1-24aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Δ24, deletion of 1-24aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-DONSON-DEW

    Plasmid

    #208802

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Bn CAAX pcDNA3

    Plasmid

    #206088

    Purpose

    Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. iCAP-BI-49-KanR

    Plasmid

    #203534

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI49 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL4.0-TERT_-100_-79

    Plasmid

    #193785

    Purpose

    luciferase reporter for duplicated TERT promoter

    Depositor

    Joseph Costello

    Article

    Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. iCAP-BI-65-KanR

    Plasmid

    #203536

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI65 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. L_NLS-mutNLS V5-Twist

    Plasmid

    #201381

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A4-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge