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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 45,354 results for: cha

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Showing: 5881 - 5920 of 45354 results

  3. Arch(D95H)-eGFP

    Plasmid
    #51081
    Purpose
    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Histidine
    Promoter
    Ubiquitin
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51081-plasmid-map-sequence-id-77349

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  9. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  11. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

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  13. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

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  28. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promoteā€¦
    Expression
    Yeast
    Available Since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

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  34. pHL 1917

    Plasmid
    #53024
    Purpose
    AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Inserts
    mCherry
    fimB (fimB E. coli)
    Use
    Synthetic Biology
    Tags
    Asp terminator, RBS(st7), and T1 terminator
    Expression
    Bacterial
    Promoter
    pCon and pLlacO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5881 - 5920 of 45354 results