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pGEX Vav1-SH2

Plasmid

#46528

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav1 (VAV1 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 661-777

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Slap-SH2

Plasmid

#46500

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap (SLA Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 61-221

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

Plasmid

#192681

Purpose

Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B

Plasmid

#120888

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Eat2-SH2

Plasmid

#46423

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Eat2 (SH2D1B Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-132

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Article

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Promoter

TEF1

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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GST eIF2beta

Plasmid

#45900

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Brk-SH2

Plasmid

#46411

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Brk (PTK6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 74-176

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSV HA eIF2beta WT

Plasmid

#45898

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Eif2s2 (Eif2s2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pGEX SHF-SH2

Plasmid

#46491

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHF (SHF Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 317-423

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHE-SH2

Plasmid

#46490

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHE

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 382-492

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHD-SH2

Plasmid

#46489

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHD (SHD Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 227-340

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/DH* (L213Q) uGFP

Plasmid

#14559

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174)/DH* (L213Q)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Rin3-SH2

Plasmid

#46476

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin3 (RIN3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 55-157

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/NSH3* (P651L) uGFP

Plasmid

#14562

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/NSH3* (P651L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Eco

Plasmid

#106276

Purpose

E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEcoCascade-IL1RNcrRNA

Plasmid

#126481

Purpose

Encodes E.coli CRISPR Type I-E crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Eco IL1RN cr26

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/SH2* (R696K) uGFP

Plasmid

#14565

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/SH2* (R696K)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only