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pEF1α-Cre-2A-mTagBFP2

Plasmid

#207746

Purpose

Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mTagBFP2

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p006-Banana-Late

Plasmid

#112251

Purpose

ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

ATF1 under osmY promoter

Expression

Bacterial

Promoter

osmY

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a LEU2

Plasmid

#61923

Purpose

Expression of K. lactis LEU2 cloned into pFA6a

Depositor

Jon Houseley, David Tollervey

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

LEU2

Use

Yeast genomic targeting

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-PFDN2

Plasmid

#31329

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

prefoldin 2 (PFDN2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-PGK-Puro

Plasmid

#207742

Purpose

Co-expression of the Cre recombinase and a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-Puro

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2-Huc-Positron-ST

Plasmid

#129265

Purpose

pan neuronal expression of soma-localized Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1B

Plasmid

#202722

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B (PIP5K1B Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mNeon

Plasmid

#207687

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
qTAG-N-Puro-3xFLAG

Plasmid

#207695

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Puro

Plasmid

#207726

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTiger-OptoSOS

Plasmid

#86439

Purpose

Single-construct blue light optogenetic Ras activator for use in Drosophila

Depositor

Jared Toettcher

Article

Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

Insert

OptoSOS

Tags

tagRFP

Expression

Insect

Promoter

Gal4-UAS

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Blast

Plasmid

#227263

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM371 - ce-unc-119 array integration

Plasmid

#182640

Purpose

Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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