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BL21(DE3) ΔserC

Bacterial strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Richard Cooley, Ryan Mehl

Publication

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
E. coli MEV20

Bacterial strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
Anti-Arl13b [N295B/66R] (Recombinant Antibody)

Antibody

#180085-rAb

Purpose

Anti-Arl13b (Mouse) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended applications

Immunohistochemistry and Western Blot

Reactivity

Mouse and Rat

Source species

Mouse

Isotype

IgG2a

Trial size

Available to purchase

Available since

March 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits
Anti-V5 [IPI-SV5-Pk1] (Recombinant Antibody)

Antibody

#218107-rAb

Purpose

Anti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.

Depositor

Institute for Protein Innovation (IPI)

Publication

Randall et al J Gen Virol. 1987 Nov;68 ( Pt 11):2769-8

Recommended applications

Western Blot

Source species

Rabbit

Isotype

IgG

Trial size

Not available to purchase

Available since

May 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits
Splicing-targeting CRISPR-Cas9 library for human lncRNAs

Pooled library

#119977

Purpose

Designed for genomic deletion screening of functional lncRNAs by targeting their splice sites.

Depositor

Wensheng Wei

Publication

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only
Human sgRNA library Gattinara in pRDA_118 backbone

Pooled library

#136986

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

Depositor

John Doench

Publication

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
RLC12

Pooled library

#163954

Purpose

Targets open reading frames and non-coding RNAs in M. tuberculosis.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Anti-HA [12CA5] (Recombinant Antibody)

Antibody

#194541-rAb

Purpose

Anti-HA recombinant mouse monoclonal antibody

Depositor

Melina Fan

Recommended applications

Immunocytochemistry and Western Blot

Source species

Mouse

Isotype

IgG2a

Trial size

Available to purchase

Available since

Feb. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits
Inzolia library in pRDA_550

Pooled library

#209552

Purpose

The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

Depositor

John Doench

Publication

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Use

CRISPR and Lentiviral

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Gouda in pRDA_118 backbone

Pooled library

#136987

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library.

Depositor

John Doench

Publication

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
Mini-human AsCpf1-based human genome-wide knockout library

Pooled library

#130630

Purpose

Knockout library targeting 16,977 genes. Each gene is targeted by an AsCpf1(AsCas12a)-based array containing 3-4 guides concatenated in one vector.

Depositor

Giulio Draetta

Publication

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only
Inzolia library in pRDA_052

Pooled library

#209551

Purpose

The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

Depositor

John Doench

Publication

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Use

CRISPR and Lentiviral

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only
RLC11

Pooled library

#163955

Purpose

Targets open reading frames and non-coding RNAs in M. smegmatis.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

Pooled library

#89640

Purpose

Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Expression

Mammalian

Use

Lentiviral

Available since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
SPECS library

Pooled library

#127842

Purpose

For performing high-throughput cell state-specific synthetic promoter screening

Depositor

Timothy Lu

Publication

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Species

Synthetic

Use

Lentiviral

Available since

Aug. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag-HA-JOSD1

Plasmid

#22547

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

JOSD1 (JOSD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG423GPD-ccdB

Plasmid

#14150

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAG424GAL-ccdB

Plasmid

#14151

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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EF1a- gfp-2a-puro

Plasmid

#129443

Purpose

expression of GFP and Puro R

Depositor

Oskar Laur

Insert

gfp-puro

Use

Lentiviral

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway Cloning

Mutation

V617F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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