We narrowed to 11,816 results for: nts
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Plasmid#71267PurposeEntry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGateway CloningTags2xGly linker and octaline synthase terminatorAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TA)
Plasmid#61513PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
TagsAIP with TA mutation (see comments), Tom20, and m…ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p11-5’_10xN_PAM-site3 (RTW572)
Pooled Library#160134PurposePlasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)DepositorSpeciesSyntheticUseCRISPRAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKAR4α
Plasmid#119919PurposeMammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imagingDepositorInserttAKAR4α
ExpressionMammalianAvailable SinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB123 - pL0_rLUC-I (CDS1)
Plasmid#123181PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plantsDepositorInsertluciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
UseLuciferase; Part for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1K97A
Plasmid#107974Purposeexpression of a dominant negative MAP Kinase KinaseDepositorInsertMKK1 K97A (MAP2K1 Human)
ExpressionMammalianMutationdominant negative; see Depositor Comments belowPromoterCMVAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL715
Plasmid#140385PurposeExpresses MphR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MphR-T
Tags6XHisExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAID5.1-C
Plasmid#145809PurposeAn all-in-one bicistronic plasmid for controlling protein degradation by AID technologyDepositorTypeEmpty backboneTagsmAIDExpressionMammalianAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBE50
Plasmid#174657PurposeCAPTURE method receiver plasmid for Bacillus cloning and heterologous expressionDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A2.0
Plasmid#99896PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRec ΔLacI
Plasmid#104575PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.DepositorInsertPTet-cas12, tetR
UseSynthetic BiologyAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
ExpressionBacterialAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry
Plasmid#173030PurposeTo express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cellsDepositorInsertSARS-CoV-2 Nucleocapsid phosphoprotein
UseLentiviralTags3xHA and IRES-mcherryExpressionMammalianPromoterGag (truncated)Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
TagsHaloTagExpressionBacterialPromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3010
Plasmid#91098PurposeModule C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoterZmUbiAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE333
Plasmid#153242PurposeM2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9-HN
Plasmid#104171PurposeCas9-WT mammalian expressionDepositorInsertCas9-WT
UseCRISPRTagsHAExpressionMammalianMutationWTPromoterCMVAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3006
Plasmid#91096PurposeModule C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoterFMV 34SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only